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Table 4 Glucose substrate saturation constants of a wildtype and a G1 knock out clone

From: Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

 

G1 k. o., μmax= 0.18 h-1

Wildtype, μmax= 0.18 h-1

D [h-1]

S [μM]

X [g L-1]

KS [μM]

S [μM]

X [g L-1]

KS [μM]

0.14

316.4

29.5

90.4

33.9

30.5

9.7

0.10

123.8

31.3

99.0

28.9

32.1

23.1

0.05

79.9

31.3

207.8

26.6

30.8

69.3

  1. Residual glucose (S), dry cell weight (X) and substrate saturation constant (KS) of a wild type and a G1 knock out (k. o.) clone at different dilution rates in chemostat cultivations.
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Microbial Cell Factories

ISSN: 1475-2859

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