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Table 3 Summary of fed batch cultivations of GCN amplified HSA expressing clones under the control of P G1 and P G6

From: Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

  

Batch end

Fed batch end

Promoter

GCN

DCW

[g L-1]

HSA

[mg L-1]

HSA/DCW

[mg g-1]

DCW

[g L-1]

HSA

[mg L-1]

HSA/DCW

[mg g-1]

PG1

11

18.9

0.2

0.0

114.0

1060.8

9.3

PG6*

11

22.5

0.3

0.0

110.8

728.7

6.6

  1. * performed in duplicates.