Table 2 Summary of fed batch cultivations of P. pastoris expressing HSA under the control of P G1 , P G6 and P GAP
| Â | Â | Batch end | Fed batch end | |||||
|---|---|---|---|---|---|---|---|---|
Promoter | GCN | DCW [g L-1] | HSA [mg L-1] | HSA/DCW [mg g-1] | DCW [g L-1] | HSA [mg L-1] | HSA/DCW [mg g-1] | % HSA/DCW of PGAP |
PG1* | 2 | 24.3 | 0.5 | 0.0 | 126.9 | 303.1 | 2.4 | 231.2 |
PG6* | 1 | 23.9 | 0.3 | 0.0 | 127.1 | 24.3 | 0.2 | 38.9 |
PGAP* | 2 | 23.8 | 10.1 | 0.4 | 123.7 | 128.2 | 1.0 | Â |
PGAP | 1 | 24.2 | 5.0 | 0.2 | 117.7 | 57.8 | 0.5 | Â |