Figure 4

Expression of secreted HSA using the novel promoters P _G1 and P _G6 in shake flask and fed batch cultivations. (A) HSA expression in shake flask screenings related to P_GAP and to the gene copy number. (B) Dry cell weight and (C) HSA titer in fed batch cultivations of double and single gene copy clones expressing under the control of P_G1 (circle, two copies), P_G6 (diamond, one copy) and P_GAP (black square, two copies and black-and-white, one copy). (D) Detail of (C) showing late batch and early fed batch phase, highlighting the different regulation properties of the promoters. Except for the single gene copy P_GAP clone, all fermentations were performed in duplicates.