Figure 3
Induction behaviour of the novel promoters. Specific eGFP fluorescence of clones expressing eGFP under the control of PG1, PG3, PG4, PG6 and PG7 in media containing different amounts of glucose. Data is related to PGAP, normalized to 1.0 at the highest glucose concentration of 20 g L-1 and plotted against the logarithmic glucose concentration (trend line calculation: four parameter logistic curve). The glucose concentration given on the x-axis refers to the glucose set point at the beginning of the cultivation (serial dilutions ranging from 20 to 0.002 g L-1). This screening setup and data processing points out relative promoter activities, thereby showing the kinetics of induction, but does not allow comparison of promoter strength.