Figure 2

Expression of eGFP under control of the novel promoters P _G1 , P _G3 , P _G4 , P _G6 , P _G7 and P _G8 . (A) Specific eGFP fluorescence in shake flask screenings related to P_GAP and to eGFP gene copy number. (B) Fed batch cultivations of single gene copy clones expressing eGFP under the control of P_GAP and P_G1. Relative eGFP expression (solid lines) and OD₆₀₀ (dashed lines) are shown over the feed time.