Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol

Li, Han; Liao, James C

doi:10.1186/1475-2859-12-4

Microbial Cell Factories

Table 3 Primers used in this study

From: Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO₂ to 1,2-propanediol

Primer Name	Sequence (5^′-3^′)	Used for plasmid
gldASpeIfwd	aggatcactagtaggagaagttaccatggaccgcattattcaatcaccgg	GYM
gldA_YqhDrev	ggtgtgcagattaaagttgttcatggtaacttctcctttattcccactcttgcaggaaac	GYM
gldA_YqhDfwd	gtttcctgcaagagtgggaataaaggagaagttaccatgaacaactttaatctgcacacc	GYM
YqhD_mgsArev	aaagtgcgagtcgtcagttccatggtaacttctcctttagcgggcggcttcgtatatacg	GYM
YqhD_mgsAfwd	cgtatatacgaagccgcccgctaaaggagaagttaccatggaactgacgactcgcacttt	GYM
mgsANotI rev	ggatcggcggccgcttacttcagacggtccgcgagataacgctga	GYM
CBSADH SpeIfwd	aggatcactagtaggagaagttaccatgaaagggtttgccatgttag	CYM
CBSADH_yqhDrev	gcagattaaagttgttcatggtaacttctcctttacaggataacaaccgc	CYM
CBSADH_yqhDfwd	gcggttgttatcctgtaaaggagaagttaccatgaacaactttaatctgc	CYM
TBSADH SpeIfwd	aggatcactagtaggagaagttaccatgaaaggttttgcaatgctgtc	TYM
TBSADH_yqhDrev	gcagattaaagttgttcatggtaacttctcctctatgctaaaatcaccac	TYM
TBSADH_yqhDfwd	gtggtgattttagcatagaggagaagttaccatgaacaactttaatctgc	TYM
pQE XhoI fwd	ttcacctcgagaaatcataaaaaatttatttgc	pZElac_his
pQE Acc65I rev	gttttcggatccgtgatggtgatggtgatgcgatcctctc	pZElac_his
his ad up rev	ggatccgtgatggtgatggtgatgcgatcc	his-gldA/CB/TB
his ad down fwd	tctagaggcatcaaataaaacgaaaggctc	his-gldA/CB/TB
his ad up_gldA fwd	gcatcaccatcaccatcacggatccatggaccgcattattcaatcaccgg	his-gldA
his ad down_gldA rev	cctttcgttttatttgatgcctctagattattcccactcttgcaggaaac	his-gldA
his ad up_CB fwd	atcaccatcaccatcacggatccatgaaagggtttgccatgttaggtatc	his-CB
his ad down_CB rev	tttcgttttatttgatgcctctagattacaggataacaaccgccttaatc	his-CB
his ad up_TB fwd	cgcatcaccatcaccatcacggatccatgaaaggttttgcaatgctgtcc	his-TB
his ad down_TB rev	ctttcgttttatttgatgcctctagactatgctaaaatcaccactggtt	his-TB
rnpBfwd	aggtgttggctcggtaaac	RT-PCR rnpB
rnpB rev	cgaagacagagggcagttatc	RT-PCR rnpB
gldAfwd	gaaaccgtagctgcccttag	RT-PCR gldA
gldA rev	ggcatgttgtgaatggtttc	RT-PCR gldA
mgsAfwd	tgacgactcgcactttacct	RT-PCR mgsA
mgsA rev	cgtgttgttccagtaacggt	RT-PCR mgsA
yqhDfwd	cgaacaaattcctcacgatg	RT-PCR yqhD
yqhD rev	tcatcagcgtttcataagcc	RT-PCR yqhD
CB fwd	ctccagtgtggtggttatcg	RT-PCR CBsADH
CB rev	ttagctgcttcaacgcaaat	RT-PCR CBsADH
TB fwd	tatggtgctaccgacatcgt	RT-PCR TBsADH
TB rev	taattgacgtttgcgatggt	RT-PCR TBsADH

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ISSN: 1475-2859

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