Table 2 Plasmids used
From: Stable expression plasmids for Streptomyces based on a toxin-antitoxin system
Plasmid | Characteristics | Reference |
|---|---|---|
pGM160 | E.coli/Streptomyces shuttle vector. Thiostrepton and gentamicin resistance. | [24] |
pGM160-YefMsl | pGM160 derivative. The xysA promoter from S. halstedii controls yefMsl expression. | [23] |
pKC796 | E.coli/Streptomyces shuttle vector. Apramycin resistance. Integrative plasmid. | [30] |
pKC796-Tox | pKC796 derivative. The xysA promoter from S. halstedii controls toxin expression. | [23] |
pKC796-TA | pKC796 derivative. The xysA promoter from S. halstedii controls TA expression. | [23] |
pXHis1 | pBluescript SK derivative. Ampicillin resistance. The xysA promoter from S. halstedii controls xys1Δ expression. | [28] |
pXHis1-Anti | pXHis1 derivative. The xysA promoter from S. halstedii controls antitoxin expression. | This work |
pN702Gem3 | E.coli/Streptomyces shuttle vector. Neomycin resistance. High-copy number. | [29] |
pN702Gem3-Anti | pN702Gem3 derivative. The xysA promoter from S. halstedii controls antitoxin expression. | This work |
pNUF5 | pN702Gem3 derivative. The pstS promoter from S. lividans controls xylanase expression. | [31] |
pNUF-Amy | pNUF5 derivative. The pstS promoter from S. lividans controls amylase expression. | This work |
pNAnti-Xyl | pN702Gem3-Anti derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls xylanase expression. | This work |
pNAnti-Amy | pN702Gem3-Anti derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls amylase expression. | This work |