Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility

Table 2 Comparison of specific activities of PhoC-GFP fusions with different linkers

Activity	Relative specific activity of IBs¹(%)			Difference in specific activity caused by linker³(%)
	PhoC-F-GFP	PhoC-R-GFP	Increase²	Soluble	IBs
Fluorescence	14.2 ± 0.1	22.0 ± 0.2	54.9	15.3 (5.70 × 10^-5)⁴	30.7 (1.76 × 10^-8)
Phosphatase	47.9 ± 1.6	58.0 ± 1.5	21.1	8.74 (1.25 × 10^-1)	10.4 (6.36 × 10^-3)
Phosphotransferase	62.8 ± 0.1	68.0 ± 1.3	8.28	1.61 (7.47 × 10^-1)	10.0 (1.15 × 10^-3)

¹ Calculated as the percentage of the specific activity of IBs relative to the corresponding specific activity of the soluble protein.
² Increase of relative specific activity of PhoC-R-GFP compared with PhoC-F-GFP as expressed by the following equation: $\frac{RS A_{PhoC - R - GFP} - RS A_{PhoC - F - GFP}}{RS A_{PhoC - F - GFP}} \times 100 %$ (RSA, relative specific activity).
³ Calculated as $\frac{|S A_{PhoC - R - GFP} - S A_{PhoC - F - GFP}|}{S A_{PhoC - F - GFP}} \times 100 %$ (SA, specific activity).
⁴p-values for the statistical significance between different linkers regarding specific fluorescence or activity are shown in parentheses (α = 0.05).

ISSN: 1475-2859