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Figure 1 | Microbial Cell Factories

Figure 1

From: A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory

Figure 1

Constructs for co-expression of AseΔ11 and AseFAR in yeast . A) represents the generation of the construct Ase∆11/CUP1p-AseFAR/GAL1p-pYEXCHT by homologous recombination through co-transformation in Saccharomyces cerevisiae of the AseFAR (yellow), which is flanked with short sequences (red) homologous to the PvuII-linearized pYEXCHT already carrying Ase∆11 (blue). B) represents how the two ORFs were fused into a single ORF by S. cerevisiae homologous recombination, generating the construct Ase∆11-FAR/CUP1p-pYEXCHT. The ORFs are flanked with a short sequence (red) homologous to the XhoI-linearized pYEXCHT on one side, as well as with a short homologous sequence interspacing the two ORFs (green) for the purpose to aid fusion into a chimeric ORF.

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Microbial Cell Factories

ISSN: 1475-2859

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