Figure 7

Analysis of His ₆ -NS3 [A,B,C] and NS5A-T-His ₆ [D] secondary structure by FT-IR and homology modeling. [A] Lower panel: FT-IR spectra of hydrated film of 10 mM phosphate buffer pH 7.4 and His₆-NS3 protein. Upper panel: Final protein spectrum obtained by subtracting the buffer spectrum. [B] Second derivative spectrum of His₆-NS3 and amide I region of the FTIR spectrum of a hydrated film of His₆-NS3 (thick grey line), and bands obtained by deconvolution. The dotted red line shows the curve fitted using the component bands. [C] Homology model of NS3/3a, built using the x-ray structure of NS3/1a as a template (PDB id: 3o8b). The protease and helicase domains are shown in red and green respectively. [D] Second derivative spectrum of NS5A-T-His₆ and amide I region of the FT-IR spectrum of a hydrated film of NS5A-T-His₆ (thick grey line), and bands obtained by deconvolution. The dotted red line shows the curve fitted using the component bands. The peak position of the spectral components along with their assignment is shown in the figure.