Table 1 Summary of the purification of cutinase-HlyAs
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude extract | 584.9 | 128497.5 | 219.7 | 100 | 1 |
Ammonium sulfate fraction | 481.7 | 113334.8 | 235.3 | 88.2 | 1.1 |
DEAE Sepharose purification | 129.9 | 51369.9 | 395.5 | 40.0 | 1.8 |
MonoQ purification | 68.8 | 30681.8 | 446.2 | 23.9 | 2.0 |