Figure 3
From: High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by Bacillus subtilis
Contribution of the RocR regulon to amylase production. B. subtilis MGB874 derivative strains in the presence (+) or absence (-) of the indicated genes were transformed with pHYK38, and then cultured in 2xL-Mal medium with shaking at 30°C. α-amylase activities were measured after 72 h cultivation. The following strains were evaluated: (a), MGB874; (b), 874DEFR; (c), 874RocR; (d), 874DEFRΔrocG; and (e), 874DEFRΔrocABC.