Figure 2From: Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastorisEnzyme purification. Hydrophobic interaction chromatography profile (A): units per fraction (___), A280nm (–·–·–·–) and gradient (········). SDS-PAGE for native and recombinant purified proteins before and after treatment with endoglicosidase H (B): High Molecular Weight Marker (HMWM), native O. piceae sterol esterase (OPE), and recombinant enzyme (OPE*). Protein bands are silver stained.Back to article page