Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering

Table 3 Fermentation time, extracellular metabolite concentrations and yields for shake-flask culture under microaerobic condition

Strain	Fermentation time, hr	Dry cell weight, g·l^-1	Residual glucose, g·l^-1	Acetaldehyde^†, g·l^-1	Yield, g·g^-1glucose
Strain	Fermentation time, hr	Dry cell weight, g·l^-1	Residual glucose, g·l^-1	Acetaldehyde^†, g·l^-1	2,3-Butanediol	Glycerol	Ethanol	Acetoin	Acetate
BY4742	40	1.349	0	0.118	0.002	0.032	0.417	0	0.017
B2C-a1^‡	56	0.787	0	0.663	0.041	0.225	0.243	0.016	0.02
B2C-a1a3	54	0.876	0	1.194	0.076	0.254	0.166	0.012	0.029
B2C-a1a5	72	0.634	1.80	1.106	0.079	0.295	0.123	0.010	0.024
B2C-a1a3a5^†	54	0.900	2.41	1.316	0.093	0.238	0.131	0.008	0.025
B2C-a1a6	57	0.740	0.09	0.493	0.046	0.265	0.231	0.010	0.002
B2C-a1a3a6^†	63	0.619	0.78	0.312	0.023	0.125	0.318	0.011	0
B2C-a1a5a6^‡	54	0.842	0	0.263	0.033	0.247	0.259	0.011	0
B2C-a1a3a5a6g2^‡	64	0.806	0	0.220	0.018	0.081	0.347	0.007	0

*All cultures were performed with an initial concentration of 20 g·l^-1 of glucose under microaerobic condition. The extracellular metabolite concentrations and yields (g metabolite produced/g glucose consumed) were determined at the time of glucose exhaustion or when the strains ceased to consume glucose. Fermentation time indicates the mean time of the end of fermentation. Residual glucose indicates the amount of glucose detected in the supernatant at the end of fermentation.
** All data correspond to the mean of four independent experiments (n = 4) unless indicated. For all data, standard error of the mean is lower than 10%.
^‡ Data correspond to the mean of three independent experiments (n = 3).
^† Data correspond to the mean of two independent experiments (n = 2).

ISSN: 1475-2859