Table 1 List of oligonucleotides used in the study for the genes amplification (restriction sites are underlined)
Primers set/gene | Primer name | Sequence (5'-3') | Expected fragment size (bp) |
|---|---|---|---|
bgl1 | Full-Beta-F | ATGGCCTCCTGGCTTGCT | 2601 |
Full-Beta-R | TCAATACGACGACGAGGGAA | ||
cbh1 | cbh1-F | AGGTCACCTTCTCCAACATC | 573 |
cbh1-R | AGAGCGGCGATTCTACGGGT | ||
F1 | AGATCGAT ATGGCCTCCTGGCTTGCTCCTG | ||
bgl1-cbh1 fusion | Fuse-REV1 | AGAAGGTGACCTTCAATACGACGACGAGGGAA | 3187 |
Fuse- F2 | GTCGTATTGAAGGTCACCTTCTCCAACATC | ||
REV2 | GGATCGAT AGAGCGGCGATTCTACGGGTTA | ||
In-Fusion | In-Fusion-F | CACAAACCGTCATCGAT ATGGCCTCCTGGCTTGCT | 3205 |
In-Fusion-R | TGCAGGTCGACATCGAT AGAGCGGCGATTCTACGGGTTA | ||
bgl1 | bgl1-Realtime-F | GCCATTCTATCCTTCGCC | 154 |
bgl1-Realtime-R | TGCCAACGCAGGCTTCAC | ||
tef1a | tef1a-Realtime-F | ACCAAGGCTGGCAAGTTC | 162 |
tef1a -Realtime-R | GACACCAGTCTCGATACG |