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Figure 5 | Microbial Cell Factories

Figure 5

From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Figure 5

Optimizations of vtPA expression in SHuffle express T7 grown in shake flask. Activity of vtPA from crude lysates of C3029, (A) grown at various temperatures, (B) induced at various growth phases, and (C) with various concentrations of IPTG. The activity of vtPA is indicated on the left Y-axis (bar). OD600nm measured at time of cell harvest is indicated on the right Y-axis (filled circle).

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Microbial Cell Factories

ISSN: 1475-2859

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