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Figure 1 | Microbial Cell Factories

Figure 1

From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Figure 1

Disulfide bond formation in the cytoplasm of SHuffle. Schematic diagram of the redox pathways in the cytoplasm of SHuffle. Dotted lines represent disabled protein interactions due to the deletion of trxB and gor. Redox state of cysteines (yellow balls) are indicated (oxidized = ball + stick; reduced = ball). (A) Protein is reduced by Grx1 or oxidized by Trx1. (B) Mis-oxidized protein is isomerized to its native correctly folded state (C) by DsbC.

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Microbial Cell Factories

ISSN: 1475-2859

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