Strain | Substrate | CDW(g/l) | PHA (wt %) | PHB (mol %) | PHHx (mol %) |
|---|
KTOYO6ΔC (phaPCJ
A.c
) | SB | 3.71 ± 0.42 | 10.0 ± 0.31 | 100 | 0 |
| | SH | 2.44 ± 0.24 | 14.64 ± 0.67 | 13.82 ± 2.30 | 86.18 ± 4.65 |
| | SB:SH (2:1) | 4.75 ± 0.20 | 32.53 ± 0.74 | 74.35 ± 4.22 | 25.65 ± 3.27 |
| | SB:SH (1:2) | 5.82 ± 0.10 | 57.80 ± 1.12 | 57.70 ± 4.29 | 42.33 ± 5.26 |
KTQQ20 | SH | 1.67 ± 0.02 | 22.03 ± 0.42 | 0 | 100 |
- SB: Sodium Butyrate, SH: Sodium hexanoate
- SB:SH (2:1): 3 gL-1 SB was added at 0 h and 12 h during the cultivation process to form the PHB block After 24 h 3 g L-1 SH was added to form the PHHx block
- SB:SH (1:2) : 3 gL-1 SB was added at 0 h and 3 g L-1 SH was fed at 12 h and 24 h of the cell growth.
- Each shake flask process continued for 48 h. 20 g/l of glucose was fed at 0 h as a nutrient in each case of block formation. PHA samples were analyzed by GC [27]
- All cells were grown in LB media for 48 h at 30 °C in the rotary shaker (HNY-2112B, Tianjin Honour Instrument Co. Ltd. China).
