Table 5 Primers used in the study
From: Production of extracellular fatty acid using engineered Escherichia coli
Primer name | Sequence (5′-3′) |
|---|---|
tesA-L | CATGCCATGGTTATGATGAAC TTCAACAATGTTTTCC |
tesA-R | CGCGGATCCTTTATGAGTCA TGATTTACTAAAGGC |
‘tesA-L | CGGAATTCGGCGGACACGTTA TTGATTCTGGG |
‘tesA-R | ACGCGTCGACTTATGAGTCATGAT TTACTAAAGGC |
fadD-IBS | AAAAAAGCTTATAATTATCCTTAGGATTCCTGCGCGTGCGCCCAGATAGGGTG |
fadD-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCCTGCGCATTAACTTACCTTTCTTTGT |
EBS2-fadD | TGAACGCAAGTTTCTAATTTCGATTAATCCTCGATAGAGGAAAGTGTCT |
fadL-IBS | AAAAAAGCTTATAATTATCCTTAGGTTACAACCTGGTGCGCCCAGATAGGGTG |
fadL-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCAACCTGACTAACTTACCTTTCTTTGT |
fadL-EBS2 | TGAACGCAAGTTTCTAATTTCGATTTAACCTCGATAGAGGAAAGTGTCT |
EBS universal | CGAAATTAGAAACTTGCGTTCAGTAAAC |