Figure 2

Optimization of pMKSal vector. Comparison of growth curve and culture-average fluorescence (Relative Fluorescence Unit per OD₅₉₀ unit) of E. coli harboring pMKSal-gfp (luxI and luxR optimized; open triangles and white bar blots for OD₅₉₀ and fluorescence, respectively), pGL506 (luxI and luxR non-optimized; grey circles and grey histogram for OD₅₉₀ and fluorescence, respectively) and pMKSal (negative control, luxI and luxR optimized; black squares and black histogram for OD₅₉₀ and fluorescence, respectively). Cell cultures were grown overnight in LB at 37°C and sub-cultured in CM medium with the appropriate antibiotic, to a final density of 0.15 OD₅₉₀. 5 replicates of each clone were incubated in 96-well plates at 30°C with shaking, monitoring the optical density and the fluorescence in a Tecan Infinite M200. Error bars show the standard deviation of the experiment.