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Table 2 Production of the active lipase LipA by the dual expression cassette plasmid systems BL21/pELipAB-LipB1a and BL21/pELipAB-LipB3a

From: Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid

Expression system BL21/

Culture supernatant

Whole cell lysate

Whole culture

Cell lysate's supernatant

Cell lysate's precipitate

 

U/g

%

U/g

%

U/g

%

U/g

%

U/g

%

pELipABa

99.9 ± 1.5

100 ± 1.5

127.5 ± 1.1

100 ± 0.8

227.4 ± 0.9

100 ± 0.4

32.4 ± 1.6

21.3 ± 1.1

119.7 ± 8.6

78.7 ± 5.6

pELipAB-LipB1a

5064.7 ± 11.2

5068.4 ± 11.2

1408.3 ± 6.9

1104.5 ± 5.4

6473.0 ± 18.0

2846.1 ± 7.9

267.9 ± 9.6

17.2 ± 0.6

1287.9 ± 49.4

82.8 ± 3.2

pELipAB-LipB3a

2930.8 ± 4.5

2932.9 ± 4.5

1352.1 ± 4.2

1060.4 ± 3.3

4282.9 ± 8.5

1883.1 ± 3.7

192.0 ± 7.0

11.8 ± 0.4

1437.9 ± 47.1

88.2 ± 2.9