Figure 1

SDS-PAGE of BL21 cell lysates using two-plasmid co-expression system. (A). pELipAB^a (1-4), pELipAB^a + pELipB1^k (6-9). Lanes (1-4) and (6-9) indicated induction by IPTG for 0, 1, 2, and 4 hours, respectively; Lane 5: standard protein. (B). pELipB3^k (1, 4, 7), pELipAB^a (2, 5, 8) and pELipAB^a + pELipB3^k (3, 6, 9). Lanes (1, 2, 3), (4, 5, 6), (7, 8, 9) indicated induction by IPTG for 0, 2, and 4 hours, respectively.