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Figure 2 | Microbial Cell Factories

Figure 2

From: L-Rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake

Figure 2

Neighbour-Joining tree showing the phylogenetic relationships of the A. nidulans rhamnosidases RhaA and RhaE. MEGA3 software [24] was used to carry out the phylogenetic analysis. Amino acid sequences were aligned and the dendogram was determined using ClustalW and the Neighbour-Joining algorithm. Bootstrap values adjacent to each internal node represent percentages of 1000 replicates. The scale bar represents aa replacements per site. Sequences from the following UniProtKB database accessions were included: A. aculeatus Q9C1M9 (RhaA), A. aculeatus Q9HFW5 (RhaB), A. kawachii A9ZT55 (Rhm78), A. nidulans C8VMJ6 (AN10277/RhaA), Bacillus sp. GL1 Q93RE8 (RhaA), Bacillus sp. GL1 Q93RE7 (RhaB), C. stercorarium Q9S3L0 (RamA), L. acidophilus Q5FJ31 (RamA), L. plantarum Q88SF8 (Ram1), L. plantarum Q88SF6 (Ram2), Pediococcus acidilactici EONEV1 (Ram), P. acidilactici EONEK0 (Ram2), Thermomicrobia sp. PRI-1686 Q93RE8 (RhmA) and Thermomicrobia sp. PRI-1686 Q93RE7 (RhmB). Sphingomonas paucimobilis RhaM (Q76LC4), a GH106 rhamnosidase, was used to root the tree.

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Microbial Cell Factories

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