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Table 1 List of S. cerevisiae strains used

From: Positive genetic interactors of HMG2 identify a new set of genetic perturbations for improving sesquiterpene production in Saccharomyces cerevisiae

Strain

Genotype

Plasmid description

Source

BY4741

Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0.

 

Research Genetics

EG60

Mat α, ura3, trp1, his3.

 

E. Golemis

EG60-01

Mat α, ura3, trp1, his3.

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM62

Mat α, ura3, trp1, his3, ubc7:: HIS5

 

This study

AM62-01

Mat α, ura3, trp1, his3, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM66

Mat α/a, PGal1-HMG2(K6R)x 2::HO, ura3, trp1, his3. Derivative of AM63 and KSY10 back-crossed 4 times.

 

This study

AM85

Mat α/a, PGal1-HMG2(K6R)x 2::HO, ura3, trp1, TDH3p-HMG2(K6R)-HIS3::leu2. Derivative of AM66

 

This study

AM88

Mat α, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3

 

This study

AM88-01

Mat α, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM89

Mat a, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3

 

This study

AM90

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X 2. Derived from cross of AM88 and AM89

 

This study

AM94

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X 2, ERG9/erg9 Derived from AM90

 

This study

AM94-01

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X 2, ERG9/erg9 Derived from AM90

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM97

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X 2, ERG9/erg9, UBC7/ubc7 derived from AM94

 

This study

AM97-01

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X 2-::leu2 ERG9/erg9, UBC7/ubc7 derived from AM94

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM102

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X 2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4 derived from AM97

 

This study

AM102-01

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X 2, ERG9/erg9, UBC7/ubc7, SSM4/ssm4 derived from AM97

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AM109

Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X 2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4, PHO86/pho86, derived from AM102

 

This study

AM109-01

Mat a/α, PGal1-HMG2(K6R)x 2:: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X 2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4, PHO86/pho86, derived from AM102

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW2

Mat a/α, ura3, his3, leu2 TRP1/trp1, DCR2/dcr2:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW3

Mat a/α, ura3, his3, leu2 TRP1/trp1, SSD1/ssd1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW4

Mat a/α, ura3, his3, leu2 TRP1/trp1, UBC7/ubc7:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW5

Mat a/α, ura3, his3, leu2 TRP1/trp1, UBI4/ubi4:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW6

Mat a/α, ura3, his3, leu2 TRP1/trp1, UBX2/ubx2:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW7

Mat a/α, ura3, his3, leu2 TRP1/trp1, DER1/der1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW8

Mat a/α, ura3, his3, leu2 TRP1/trp1, SPF1/spf1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW9

Mat a/α, ura3, his3, leu2 TRP1/trp1, NPL4/npl4:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW10

Mat a/α, ura3, his3, leu2 TRP1/trp1, SKM1/skm1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW11

Mat a/α, ura3, his3, leu2 TRP1/trp1, CUE1/cue1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW12

Mat a/α, ura3, his3, leu2 TRP1/trp1, SSM4/ssm4:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW13

Mat a/α, ura3, his3, leu2 TRP1/trp1, DOA1/doa1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW14

Mat a/α, ura3, his3, leu2 TRP1/trp1, HMG1/hmg1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW15

Mat a/α, ura3, his3, leu2 TRP1/trp1, CHO2/cho2:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW16

Mat a/α, ura3, his3, leu2 TRP1/trp1, GRE3/gre3:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW17

Mat a/α, ura3, his3, leu2 TRP1/trp1, WHI2/whi2:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW18

Mat a/α, ura3, his3, leu2 TRP1/trp1, YTA7/yta7:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW19

Mat a/α, ura3, his3, leu2 TRP1/trp1, PHO86/pho86:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW20

Mat a/α, ura3, his3, leu2 TRP1/trp1, RIC1/ric1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW21

Mat a/α, ura3, his3, leu2 TRP1/trp1, GAS1/gas1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW22

Mat a/α, ura3, his3, leu2 TRP1/trp1, ADH1/adh1:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW23

Mat a/α, ura3, his3, leu2 TRP1/trp1, GDH1/gdh1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW24

Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD1/hrd1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW1

Mat a/α, ura3, his3, leu2 TRP1/trp1

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMW25

Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD3/hrd3:: G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

ΑΜΗ9

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH13

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DCR2/dcr2::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH15

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SSD1/ssd1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH16

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBC7/ubc7::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH17

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBI4/ubi4::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH18

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBX2/ubx2::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH19

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DER1/der1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH20

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS3::leu2, SPF1/spf1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH21

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, NPL4/npl4::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH22

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SKM1/skm1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH23

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, CUE1/cue1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH24

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SSM4/ssm4::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH25

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DOA1/doa1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH26

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, HMG1/hmg1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH27

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS3::leu2, CHO2/cho2::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH28

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, GRE3/gre3::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH29

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, WHI2/whi2::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH30

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, YTA7/yta7::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH31

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, PHO86/pho86::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH32

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, RIC1/ric1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH33

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, GAS1/gas1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH34

Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, BFR1/bfr1::G418

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH47

Mat a/α, ura3, his3, leu2 TRP1/trp1, DCR2/dcr2:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH49

Mat a/α, ura3, his3, leu2 TRP1/trp1, SSD1/ssd1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH50

Mat a/α, ura3, his3, leu2 TRP1/trp1, ubc7:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH51

Mat a/α, ura3, his3, leu2 TRP1/trp1, UBI4/ubi4:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH52

Mat a/α, ura3, his3, leu2 TRP1/trp1, UBX2/ubx2:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH53

Mat a/α, ura3, his3, leu2 TRP1/trp1, DER1/der1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH54

Mat a/α, ura3, his3, leu2 TRP1/trp1, SPF1/spf1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH55

Mat a/α, ura3, his3, leu2 TRP1/trp1, NPL4/npl4:: G418,UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH56

Mat a/α, ura3, his3, leu2 TRP1/trp1, SKM1/skm1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH57

Mat a/α, ura3, his3, leu2 TRP1/trp1, CUE1/cue1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH58

Mat a/α, ura3, his3, leu2 TRP1/trp1, SSM4/ssm4:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH59

Mat a/α, ura3, his3, leu2 TRP1/trp1, DOA1/doa1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH60

Mat a/α, ura3, his3, leu2 TRP1/trp1, HMG1/hmg1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH61

Mat a/α, ura3, his3, leu2 TRP1/trp1, CHO2/cho2:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH62

Mat a/α, ura3, his3, leu2 TRP1/trp1, GRE3/gre3:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH63

Mat a/α, ura3, his3, leu2 TRP1/trp1, WHI2/whi2:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH64

Mat a/α, ura3, his3, leu2 TRP1/trp1, YTA7/yta7:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH65

Mat a/α, ura3, his3, leu2 TRP1/trp1, PHO86/pho86:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH66

Mat a/α, ura3, his3, leu2 TRP1/trp1, RIC1/ric1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH69

Mat a/α, ura3, his3, leu2 TRP1/trp1, ADH1/adh1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH70

Mat a/α, ura3, his3, leu2 TRP1/trp1, GDH1/gdh1::G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH74

Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD1/hrd1::G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH76

Mat a/α, ura3, his3, leu2 TRP1/trp1 UBC7/ubc7:: HIS5 Control Strain

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH77

Mat a/α, ura3, his3, leu2 TRP1/trp1 HRD3/hrd3:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMH78

Mat a/alpha, ura3, his3, leu2 TRP1/trp1 BFR1/bfr1:: G418, UBC7/ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMD21

Mat α, ura3, his3, leu2, pho86:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMD14

Mat α, ura3, his3, leu2, ssm4:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMD5

Mat α, ura3, his3, leu2, ssd1:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMD7

Mat α, ura3, his3, leu2, ubi4:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

AMD33

Mat α, ura3, his3, leu2, hrd3:: G418, ubc7:: HIS5

pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126

This study

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