Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

Table 2 Linear covalently closed (lcc) plasmid confers reduced integration frequency

¹ R-cells are all W3110 derivatives expressing recombinase gene at 42°C the recombinase gene is fully induced . R-cells carry the λ int expression plasmid pAH153.
² pAH120 plasmid carrying λ attP.
³ Mean of minimum 3 trials. IF is expressed as fraction of integration frequency of parent strain, W3110.

ISSN: 1475-2859