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Figure 5 | Microbial Cell Factories

Figure 5

From: Rationally re-designed mutation of NAD-independent l-lactate dehydrogenase: high optical resolution of racemic mandelic acid by the engineered Escherichia coli

Figure 5

Time course of the kinetic resolution of racemic mandelic acid by whole cells of E. coli expressing V108A l -iLDH. (♦) l-mandelic acid; (▪) d-mandelic acid; (□) benzoylformic acid. Accurate concentrations of benzoylformic acid and mandelic acid in the reaction mixture were analyzed by HPLC at the indicated times. Values are the mean ± SD of 3 separate determinations.

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Microbial Cell Factories

ISSN: 1475-2859

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