The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG

Table 2 Sequence coverage of Msp1.Tryptic peptides were registered by RP-LC-ion trap-MS/MS. Filled circles indicate hexoses

(Glyco)peptide	Registered signal (*m/z*)	Calculated mass/determined mass	Mascot score
S₃₃K₄₀	Not detected	841.42/-
S₄₁K₅₆	595.48 [M+3H]³⁺	1783.41/1783.44	60
R₅₇	Not detected	174.11/-
Y₅₈K₈₁	720.90 [M+2H]²⁺	1439.78/1439.80	90
T₇₁K₈₁	641.78 [M+2H]²⁺	1281.54/1281.56	68
V₈₂K₉₈	639.34 [M+3H]³⁺	1914.99/1915.02	46
T₉₉K₁₅₀	Not detected	5170.51/-
T₉₉K₁₅₀ ●●	1374.56 [M+4H]⁴⁺ 1099.81 [M+5H]⁵⁺ 916.81 [M+6H]⁶⁺	5494.62/5494.35	Manually assigned
T₉₉K₁₅₀ ●●●	1415.06 [M+4H]⁴⁺ 1132.31 [M+5H]⁵⁺ 943.75 [M+6H]⁶⁺	5656.67/5656.43	Manually assigned
T₉₉K₁₅₀ ●●●●	1455.55 [M+4H]⁴⁺ 1164.69 [M+5H]⁵⁺ 970.75 [M+6H]⁶	5818.72/5818.38	Manually assigned
T₉₉K₁₅₀ ●●●●●	1496.25 [M+4H]⁴⁺ 1197.13 [M+5H]⁵⁺ 997.88 [M+6H]⁶⁺	5980.77/5980.98	Manually assigned
N₁₅₁R₁₉₃	1168.50 [M+4H]⁴⁺	4669.25/4669.0	Manually assigned
L₁₉₄R₂₀₃	509.24 [M+2H]²⁺	1016.46/1016.48	49
F₂₀₄R₃₇₈	Not detected	15931.57/-
Q₃₇₉K₃₈₁	Not detected	345.20/-
I₃₈₂K₄₀₂	752.78 [M+3H]³⁺	2255.32/2255.34	92
G₄₀₃K₄₈₁	Not detected	8257.93/-
Y₄₈₂L₄₉₈	989.46 [M+2H]²⁺	1976.90/1976.92	90

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