Figure 8
From: The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
Relationship between Msp1 glycosylation and cellular location. LGG wild-type was grown for 24 h in AOAC medium and proteins were isolated from spent culture supernatant, the cell wall and cytosol fraction as described in Materials and Methods. Samples were run on SDS-PAGE and blot with Msp1 antiserum or ConA lectin. For comparison, non-glycosylated recombinant Msp1 (rec. Msp1) was included.