Figure 7
From: The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
Impact of Msp1 glycosylation on peptidoglycan hydrolase activity. The cell wall hydrolyzing capacity of glycosylated (glyc.) and recombinant (rec.) non-glycosylated Msp1 was determined with msp1 mutant CMPG10200 cells as substrate. Supernatant (SN) samples from LGG wild-type (WT) and the msp1 mutant CMPG10200 were also loaded on the zymogram gels. The gels were incubated overnight at 37°C in phosphate buffer containing 1 mM DTT supplemented with the different Msp1 samples and visualized by methylene blue staining as described in materials and methods.