Figure 5
From: The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
Comparative analysis of the glycosylation of Msp1 in L. rhamnosus and L. casei strains. (A) Supernatant proteins from different L. rhamnosus and L. casei strains were run on SDS-PAGE and stained with Sypro® Ruby, and transferred to PVDF membranes for Western blot analysis with Msp1 antiserum or ConA lectin. (B) Multiple alignment of part of the Msp-1 like proteins in different L. casei and L. rhamnosus strains. The corresponding msp1 genes were obtained by sequencing after PCR as described in the Methods section. The glycosylation site on the S106-S107 dipeptide determined by MS/MS for LGG is indicated with asterisks.