Figure 3
From: The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
Analysis of tryptic Msp1 glycopeptides by mass spectrometry. (A) Sum mass spectrum of the elution range of tryptic glycopeptides T99K150 carrying two to five hexose residues. Glycopeptides were detected with four, five, and six charges. (B) Ion trap-MS/MS of T99K150 carrying four hexoses ([M+5H]5+ at m/z 1165.4). The MS/MS spectrum was acquired by nano-LC-MS/MS of the total tryptic digest of Msp 1. The Ser-Ser motif which was found to be substituted by hexoses is highlighted in blue. Filled circle indicates hexose.