Figure 1From: Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicumpTGR platform features. (A) Map of the generic pTGR synthetic plasmid where all parts are flanked by a standard set of unique restriction sites. (B) The two types of constructions for the expression of multiple genes. Sequential insertion of cassettes containing RBS-ORF for the assembly of operons or cassettes comprising Promoter-RBS-ORF-transcriptional terminator for the assembly of gene clusters.Back to article page