Table 1 Fermentation conditions and protein yields for VCL and VCLH constructs
Construct | Process | Pre- induction phase | Induction temperature (°C) | Induction phase | Final OD (600 nm) | Wet cell paste (g/L) | Volumetric productivity (g/L) | Specific productivity (g/OD600 nm) |
|---|---|---|---|---|---|---|---|---|
VCL | Flask Complex medium | 24 h at 37°C | 25 | 25°C for 10 h 15°C for 14 h | 6.4 | 9.7 | 0.3 | 0.05 |
VCL | Flask Defined medium | 24 h at 37°C | 25 | 25°C for 10 h 15°C for 14 h | 5.6 | 8.3 | 0.2 | 0.04 |
VCL | Batch Defined medium | 5 h at 37°C | 25 | 25°C for 7 h | 5.5 | 5.3 | 0.7 | 0.13 |
VCLH | Batch Defined medium | 5 h at 37°C | 25 | 25°C for 7 h | 4.3 | 5.8 | 1.0 | 0.23 |
VCLH | Fed batch Defined medium | 24 h at 37°C | 25 | 25°C for 10 h | 106 | 108 | 8.2 | 0.08 |
Fed batch Defined medium | 24 h at 37°C | 25 | 25°C for 10 h | 88 | 113 | 9.5 | 0.11 | |
VCL | Fed batch Defined medium | 24 h at 37°C | 15 | 15°C for 24 h | 65 | 112 | 10.0 | 0.15 |
VCL | Fed batch Defined medium | 24 h at 37°C | 25 | 25°C for 10 h 20°C for 5 h 15°C for 9 h | 96 | n.d. | 19.3 | 0.20 |
VCL | Fed batch Defined medium | 24 h at 37°C | 25 | 25°C for 10 h 20°C for 5 h 15 °C for 9 h | 105 | 137 | 13.0 | 0.12 |
VCL | Fed batch Defined medium | 24 h at 37°C | 25 | 25°C for 10 h 20°C for 5 h 15°C for 9 h | 144 | 148 | 19.0 | 0.13 |