“Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

Table 3 Bacterial strains used in this study

Strains	Endogenous methylation		Particular phenotypes	Source
	dam	dcm
Escherichia coli
JM109	+	+		New England Biolabs GmbH
BL21 (DE3)	+	-		New England Biolabs GmbH
GM33	-	+	dam-3	M. G. Marinus
C2925	-	-	dcm-6/dam 13::Tn9	New England Biolabs GmbH
Lactic acid bacteria
L. plantarum CD032			wilde type strain	stable grass silage accession number BT6146*
L. plantarum CD033			wilde type strain	stable grass silage accession number BT6326*
L. buchneri CD034			wilde type strain	stable grass silage accession number BT6327*
E. faecium CD036			wilde type strain	stable grass silage accession number BT6329*
L. lactis MG1363			Plasmid free derivative of SH4109	[45]
L. plantarum DSM20174			type strain	[39]

* deposited in the strain collection of the Institute of Molecular Biotechnology, Graz, University of Technology, A-8010, Graz, Austria.

ISSN: 1475-2859