Figure 1

Schematic diagram of the co-expression system to obtain in vivo tyrosine-modified MAP. (A) pACYC-Tyr-438 harboring two genes of tyrosinase and ORF 438 was used for functional expression of tyrosinase (left vector map), and pET-fp151 was used for expression of fp-151 (right vector map). (B) The two recombinant plasmids were introduced into E. coli BL21 (DE3) for co-expression of fp-151, tyrosinase, and ORF 438. Through this co-expression strategy, in vivo tyrosine-modified MAP can be obtained without additional in vitro modification.