Figure 4
From: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli
Western blot showing distribution of SefA and H:gm from pDT1 in cellular fractions. Detection through an anti-His6 antibody conjugated with horseradish peroxidase. Lanes 1 and 6: Size marker. Lanes 2 and 7: Outer membrane fraction of pAIDA1 without passenger (positive control). Lane 3: pDT1-SefA soluble fraction. Lane 4: pDT1-SefA inner membrane fraction. Lane 5: pDT1-SefA outer membrane fraction. Lane 8: pDT1-H:gm soluble fraction. Lane 9: pDT1-H:gm inner membrane fraction. Lane 10: pDT1-H:gm outer membrane fraction.