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Table 2 Effect of organic solvents and cations on activity of Est_p1

From: A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea

Solvent

Relative activity (%)

Cations

Relative activity (%)

 

0.5 mM

1 mM

2 mM

 

0.5 mM

1 mM

2 mM

GITC

113 ± 0.8

104 ± 1

93.7 ± 8

Mg2+

97.6 ± 12

101 ± 0.03

86.7 ± 3

PMSF

104 ± 2

110 ± 3

99.5 ± 8

Ca2+

96.3 ± 3

86.5 ± 1

55.1 ± 3

PVPP

85.6 ± 3

73.5 ± 3

58.6 ± 3

Mn2+

78.0 ± 6

85.5 ± 3

63.5 ± 2

SDS

99.5 ± 2

71.5 ± 0.6

30.0 ± 1

Co2+

82.3 ± 6

79.6 ± 14

12.0 ± 2

Thiourea

105 ± 1

95.6 ± 6

95.3 ± 0

Ni2+

54.4 ± 5

39.1 ± 7

24.4 ± 4

Urea

102 ± 0.6

105 ± 2

101 ± 2

Zn2+

24.1 ± 10

24.6 ± 5

24.4 ± 11

EDTA

110 ± 18

113 ± 6

108 ± 5

Cu2+

19.1 ± 0.1

11.3 ± 2

4.11 ± 1