Figure 4From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectorsPrimer extension. (A) Primer extension of P mtlA in pKAM12. The procedure has been explained in Methods. A, C, G, and T represent the dideoxynucleotide triphosphates used for the pKAM1 sequencing, and the + and - display the primer extension reaction of induced and uninduced sample, respectively. (B) Primer extension of P mtlR in pKAM18 and DNA sequencing reaction.Back to article page