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Table 2 Primers used for gene cloning in this study

From: Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid

Primer Name

Nucleotide Sequence

Restriction site

P148L-F

5'-TTAGATCTGAATAGCCCGCAATACCTGGGC- 3'

 

P148L-R

5'-GCTATTCAGATCTAACGCTTCCGTCGCCAGTGG - 3'

 

aroFSacI-F

5'-AACGAGCTC ACCGGAAAGTCCTCGGGCATAAG - 3'

Sac I

aroFSacI-R

5'-AACGAGCTC CGACTTCATCAATTTGATCGCGTAA - 3'

Sac I

pheA-M-F

5'-GGGAATTC TATGACATCGGAAAACCCGTTAC - 3'

Eco R I

pheA-M-R

5'- ATCCGGAAGCTT TTCATCAGG - 3'

Hin d III

pheA-MN-FM

5'- AACAAGCCTGTGCGCTGG - 3'

 

pheA-M-RM

5'- TCAACCAGCGCACAGGCTTGTTGC - 3'

 

PTacpheA*-F

5'- GATCCGAAGCTT ATCGACTGCACG - 3'

Hin d III

PTacpheA*-R

5'- AGTCGAC GCTTTTCATCAGGTTGG - 3'

Sal I

lacIq-F

5'- CGCTAGC CCTGACGGGCTTGTCTG - 3'

Nhe I

lacIq-R

5'- CGCTAGC TTCCGATGGCTGCCTG - 3'

Nhe I

hmaSAo-F

5'- CGCAT ATG CAGAATTTCGAGATCGACTAC - 3'

Nde I

hmaSAo-R

5'- CAAGCTT AACGTACGTCATCGCCG - 3'

Hin d III

hmo-F

5'-GATATACC ATG G GCAGCAGCCATC-3'

Nco I

hmo-R

5'-CGGTACC TGGTCATCCGTGGCTCCTG-3'

Kpn I

pETrbs+hmaSAo-F

5'- CCGTCGAC AAATAATTTTGTTTAACTTTAAG - 3'

Sal I

pETrbs+hmaSAo-R

5'- CGGCCGGATCC TTGAAGATCTC - 3'

Bam H I

dmd-F

5'-CCAT ATG CCTCGCCCTCGCGTC-3'

Nde I

dmd-R

5'-GGAATTC AGTAGGCGCGAAAAGCG-3'

Eco R I

pETrbs+dmd-F

5'-CGGTACC TTTGTTTAACTTTAAGAAGG-3'

Kpn I

pETrbs+dmd-R

5'-ACTGCAG TGGTGGTGGTGGTGGTGCT-3'

Pst I

pTrcSD-F

5'-AGGATCC AAATCACTGCATAATTCG-3'

Bam H I

pTrcSD-R

5'-TGGATCC GTTATTGTCTCATGAGCG-3'

Bam H I

  1. Underlined text denotes the restriction site sequences, and bold type indicates the start condons.