Figure 6

Cleavage of the thioredoxin-Bbr_0140 fusion protein by enterokinase. (A) Protein gel analysis of purified TrxA-Bbr_0140 incubated for 16 h at 20°C without enterokinase (lane 1), incubated for 16 h at 37°C with enterokinase (lane 2), incubated for 16 h at 37°C with enterokinase and 1% (v/v) SDS (lane 3), incubated for 16 h at 20°C with enterokinase and 1% (v/v) SDS (lane 4). (B) Protein gel analysis of purified Bbr_0140 after cleavage (lane1) and after cleavage and subsequent purification (lane 2).