Figure 5

Vitamin B ₁₂ production by L. reuteri strains in commercial Vitamin B ₁₂ -assay medium supplemented with glycerol. Bars represent average values along with standard deviation (error bars) of at least two independent biological replicates measured in triplicate using strains 6475 (parent strain), 6475::pocR (PocR deficient mutant), 6475::pocR pJKS100 (PocR deficient mutant transformed with empty plasmid), 6475::pocR pJKS101 (PocR deficient mutant complemented with putative pocR of 6475 under control of its native promoter), JCM1112 pNZ7021 (type strain transformed with empty plasmid) and JCM1112 pNZ7748 (type strain carrying the lreu_1750 overexpression).