Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures

Table 1 Mixtures of PFD used during experiments and total content of oxygen in the liquid oxygen carrier

Symbol	Composition of PFD mixture (per 1.0 ml)	Content of oxygen [μM O₂(ml^-1PFD)]
		from air	from pure oxygen	total
PFD _air	1.0 ml PFD_air	4.00	0	4.00
PFD _20%O2	0.8 ml PFD_air + 0.2 ml PFD_O2	3.20	3.84	7.04
PFD _40%O2	0.6 ml PFD_air + 0.4 ml PFD_O2	2.40	7.68	10.08
PFD _60%O2	0.4 ml PFD_air + 0.6 ml PFD_O2	1.60	11.52	13.12
PFD _80%O2	0.2 ml PFD_air + 0.8 ml PFD_O2	0.80	15.36	16.16
PFD _O2	1.0 ml PFD_O2	0	19.20	19.20

The oxygen concentration in PFD was calculated based on data published by Costa Gomez et al. [29] and Amaral et al. [31].

ISSN: 1475-2859