Figure 1

The comparison of growth (OD ₆₀₀ )(A) and ADH amounts (B and C) for batch cultures of E. coli RB791 pAdh on LB-medium supplemented with PFD _40%O2 and or without PFD (negative control, marked as "PFD_"). Cultured cells were induced by IPTG at various times (1.5, 2.0, 2.5, and 3.0 h after inoculation). OD₆₀₀ values were measured at the time of induction and after 3 and 9 h after induction. SDS-PAGE analysis of soluble (B) and insoluble (C) protein fractions: t - time of IPTG induction; PFD +/- - cultures supplemented (+) with PFD_40%O2 or control cultures (-) without PFD; arrows - heterologous alcohol dehydrogenase (ADH).