Figure 1
From: Streamlined protein expression and purification using cleavable self-aggregating tags
Strategies of quick protein expression and purification using self-cleaving aggregation tags. (A) Npro fusion technology, fusions expressed as inclusion bodies are separated, refolded, and cleaved, followed by a polishing step to remove soluble Npro. (B) The ELP system and (C) this work, the self-cleaving aggregation tags are based on the combination of a self-cleavable intein and a self-aggregating peptide (ELP) or a self-assembling peptide (18A or ELK16). T/S: temperature or salt-mediated.