Figure 6

The new strains are robust and allow further improvements through plasmid-driven gene expression. (A) The robustness of the newly developed AM66, AM68, AM70, AM78 strains were tested in a growth assay in galactose based media. No difference with wild type EG60 cells is observed; (B) AM78-01 cells (triangle), AM65-01 cells (square), and parental EG60-02 (rhomboid) cells induced for protein expression were sampled by SPME daily over a 19 day period and monitored for cineole production; (C) Co-expression in AM78-05 cells of IDI1 from two high copy number plasmids did not cause higher cineole production, whereas the co-expression of IDI1 with HMG2 (K6R) in AM78-07 doubled the yield.