Table 1 Dye Screening for MP estimation
Dye | Increasing fluorescence according to cell status | Concentration range (μM) | Incubation time range (min) | Maximum difference to depolarized cells with CCCP | Influence on | ||
|---|---|---|---|---|---|---|---|
|  |  |  |  | Concentration (μM) | Incubation time t (min) | SS (% ± SD) | EV (% ± SD) |
DiOC 2 (3) | Red (675 nm)/green (525 nm) ratio, polarized | 4-80 | 0-43 | 14 | 5 < t < 27 | -18 (±8) | -19 (±5) |
DiOC 6 (3) | Green (525 nm), polarized | 0.015-0.4 | 0-36 | 0.365 | 5 < t <28 | -14 (±6) | -54 (±5) |
DiBAC 4 (3) | Green (675 nm), depolarized | 0.02-3 | 0-43 | 2.1 | 2 < t <4 | -47 (±8) | -84 (±8) |