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Figure 2 | Microbial Cell Factories

Figure 2

From: Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

Figure 2

Design of the plasmids for pre- and co-expression of Erv1p and/or a protein disulfide isomerase. Co-expression is based on a polycistronic vector based on pET23 with a T7 promoter/terminator and IPTG induction. Pre-expression is based on cloning the Erv1p +/- the disulfide isomerase into an arabinose inducible system cloned into pLysS. Since the vector map for pLysSRARE is not available we were unable to clone into this vector and hence when using a Δgor ΔtrxB strain the co-expression studies are done in an origami rather than rosetta-gami background.

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Microbial Cell Factories

ISSN: 1475-2859

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