A GH51 α-l-arabinofuranosidase from Talaromyces leycettanus strain JCM12802 that selectively drives synergistic lignocellulose hydrolysis

Background The development of sustainable technologies for plant cell wall degradation greatly depends on enzymes with hydrolytic activities against carbohydrates. The waste by-products of agricultural cereals are important biomass sources because they contain large amounts of saccharides. Achieving efficient debranching and depolymerization are two important objectives for increasing the utilization of such renewable bioresources. GH51 α-l-arabinofuranosidases are important in biomass pretreatment because they act synergistically with other enzymes during hemicellulose hydrolysis. Results A GH51 α-l-arabinofuranosidase from Talaromyces leycettanus JCM12802 was heterologously expressed in Pichia pastoris GS115 and characterized. The recombinant α-l-arabinofuranosidase, TlAbf51, showed an optimum temperature and pH of 55–60 °C and 3.5–4.0, respectively, and remained stable at 50 °C and pH 3.0–9.0. TlAbf51 showed a higher catalytic efficiency (5712 mM−1 s−1) than most fungal α-l-arabinofuranosidases towards the substrate 4-nitrophenyl-α-l-arabinofuranoside. Moreover, TlAbf51 preferentially removed 1,2- or 1,3-linked arabinofuranose residues from arabinoxylan and acted synergistically with the bifunctional xylanase/cellulase TcXyn10A at an activity ratio of 5:1. The highest yields of arabinose and xylooligosaccharides were obtained when TlAbf51 was added after TcXyn10A or when both enzymes were added simultaneously. High-performance anion-exchange chromatography analyses showed that (i) arabinose and xylooligosaccharides with low degrees of polymerization (DP1–DP5) and (ii) arabinose and xylooligosaccharides (DP1–DP3) were the major hydrolysates obtained during the hydrolysis of sodium hydroxide-pretreated cornstalk and corn bran, respectively. Conclusions In contrast to other fungal GH51 α-l-arabinofuranosidases, recombinant TlAbf51 showed excellent stability over a broad pH range and high catalytic efficiency. Moreover, TlAbf51 acted synergistically with another hemicellulase to digest arabino-polysaccharides. These favorable enzymatic properties make TlAbf51 attractive for biomass pretreatment and biofuel production. Electronic supplementary material The online version of this article (10.1186/s12934-019-1192-z) contains supplementary material, which is available to authorized users.


Background
The large amounts of by-products produced during the machining of agricultural cereals, such as straw, stover, and husks, are important resources for biofuel production [1]. Lignocellulosic biomass, as the most abundant renewable bioresource, is derived from plant cell walls and is mainly comprised of cellulose, hemicellulose, and lignin, among which cellulose and hemicellulose are the first and second most abundant polysaccharides on Earth [2,3]. Because hemicellulose is concatenated with cellulose via hydrogen bonds and is chemically cross-linked with lignin, it is naturally resistant to digestion by cellulases. Thus, hemicellulose degradation can help cellulases access cellulose, resulting in more efficient cellulose utilization [4,5]. Therefore, a more efficient means of enzymatic hemicellulose depolymerization in the biofuel and biorefinery industries is desired.

Gene cloning and sequence analysis
An Abf, designated here as TlAbf51, was isolated from the thermophilic T. leycettanus JCM12802. The fulllength chromosomal and cDNA sequences of Tlabf51 (GenBank accession no. MK734377) consisted of 2362 and 1887 base pairs, respectively. Eight introns interrupted the cDNA sequence and the mature protein contained 628 residues with a calculated molecular mass of 67.2 kDa. Seven putative N-glycosylation sites and four O-glycosylation sites were identified within the deduced TlAbf51 sequence by NetNGlyc Server analysis. The deduced amino acid sequence of TlAbf51 shares the highest identity of 79.9% with the glycoside hydrolase of Aspergillus ellipticus CBS 707.79 and 28.1% sequence identity with the known crystal structure of Abf from Bifidobacterium longum (2Y2W), demonstrating that Tlabf51 is a novel Abf gene.

Expression and purification of recombinant TlAbf51
Recombinant TlAbf51 was expressed in the P. pastoris GS115 system and secreted into the culture medium. Significant Abf activities were observed in shake tube cultures against 4-nitrophenyl-α-l-arabinofuranoside. After large-scale cultivation and purification, the electrophoretic homogeneity of recombinant TlAbf51 was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis (Additional file 1). To verify the target protein, liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS) was conducted to identify the band. Five peptides corresponding to the sequence of recombinant TlAbf51 and no other peptides were detected (Additional file 2). These results confirmed the purity of the band and the identity of TlAbf51.

Biochemical characterization
For enzyme characterization, 4-nitrophenyl-α-larabinofuranoside was used as a substrate. TlAbf51 was optimally active at pH 3.5-4.0 and showed > 40% of its peak activity at pH 2.5-5.0 (Fig. 1a). The enzyme exhibited good stability over a wide pH range, maintaining > 70% of its maximum activity after incubation at pH 3.0-9.0, 37 °C for 1 h (Fig. 1a). The optimal temperature for TlAbf51 activity was 55-60 °C and the enzyme was active over a temperature range of 20-70 °C at pH 3.5 (Fig. 1b). The enzyme showed good thermostability, and > 90% residual activity was retained after 30-min incubation at 50 °C (Fig. 1b). The T 50 value of TlAbf51, i.e., the temperature corresponding to 50% of the peak activity following a 30-min incubation period, was determined to be 55 °C.

Synergistic effect of TlAbf51 and TcXyn10A on wheat arabinoxylan degradation
To improve the efficiency of xylan hydrolysis, the hydrolysis efficiency of water-soluble wheat arabinoxylan by TlAbf51 and bifunctional xylanase/cellulase TcXyn10A, tested individually or in combination, were analyzed by high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Compared to the hydrolysis products generated by TlAbf51 or TcXyn10A alone, all enzyme combinations showed significant synergistic effects on wheat arabinoxylan degradation, producing markedly higher levels of arabinose and xylooligosaccharides. After 12-h incubation of TlAbf51 with 0.5% wheat arabinoxylan, the concentration of arabinose reached 184.3 mg/L (Fig. 3a). When incubated with 0.5 U TcXyn10A, the levels of xylooligosaccharides (xylose, xylobiose, and xylotriose) reached 451.4 mg/L, although small amounts of arabino-branched xylooligosaccharides were observed (Fig. 3b). Adding TlAbf51 first, followed by TcXyn10A, resulted in a significant increase in the production of arabinose and xylooligosaccharides (963.6 mg/L) as compared to adding TcX-yn10A alone (Fig. 3c). When TcXyn10A was added first, followed by TlAbf51, larger amounts of arabinose, xylotetraose, and xylopentaose were released (1455.9 mg/L; Fig. 3d). The greatest synergy was found after simultaneously incubating wheat arabinoxylan with TlAbf51 and To further examine the synergistic effects of TlAbf51 and TcXyn10A, the released reducing saccharides from water-soluble wheat arabinoxylan by simultaneous incubation with both enzymes were analyzed by the 3,5-dinitrosalicylic acid (DNS) method. TlAbf51 treatment alone liberated reducing sugars from wheat arabinoxylan (Fig. 4a). Higher concentrations of reducing sugars were observed when TlAbf51 and TcXyn10A were combined at different activity ratios compared to treatment with TcXyn10A alone. These results suggest that TlAbf51 acted synergistically with TcXyn10A. The highest degree of synergy was obtained when TcXyn10A and TlAbf51 were added at an enzyme-activity ratio of 1:5, corresponding to a synergy score of 1.47 (Fig. 4b).

Hydrolysis of sodium hydroxide-pretreated cornstalk and corn bran with TlAbf51 and TcXyn10A
Using sodium hydroxide-pretreated cornstalk and corn bran as respective substrates, TlAbf51 and TcXyn10A were further tested for their ability to hydrolyze lignocellulose. The reducing sugars in the hydrolysis products obtained after 3-h treatment of sodium hydroxide-pretreated cornstalk and corn bran with TlAbf51 and TcX-yn10A individually or combined at the activity ratio of 1:5 (0.5 and 2.5 U) were analyzed by DNS method (Fig. 5a). TlAbf51 and TcXyn10A showed significant synergistic effects. For this enzyme-activity ratio, the maximum amounts of oligosaccharides (corresponding to 37.9% hydrolysis of the cornstalk) were obtained after a 24-h hydrolysis of cornstalk ( Fig. 5b; Additional file 3). Xylotriose was the major oligosaccharide produced, followed by arabinose, xylobiose, and low amounts of xylose, xylotetraose, and xylopentaose. Interestingly, the enzymes dramatically increased the oligosaccharide levels to 2.8 g/L (corresponding to 56.2% hydrolysis of the corn bran) during the hydrolysis of corn bran ( Fig. 5c; Additional file 4). Among the released sugars, the most abundant were arabinose, xylose, xylobiose, and xylotriose, which differed from the hydrolysis products of cornstalk. After 36-h hydrolysis, 1.65 g/L arabinose was obtained from corn bran, which was ~ 2.8-fold more than that obtained from cornstalk.

Effect of TlAbf51 on arabinose release from cellulosic biomass
Cornstalk and corn bran were selected as cellulosic biomass, and the effect of TlAbf51 and the commercial complex enzyme ULTRAFLO XL on arabinose release was compared. As shown in Additional files 5 and 6, the effect of TlAbf51 on arabinose release from cornstalk was comparable with that of ULTRAFLO XL, but significantly weaker than that of ULTRAFLO XL in corn bran. Our The amount of arabinose released by TlAbf51 with each of the three substrates based on the chromatographic peak areas of arabinose shown in a. Sugar release was quantified (right panel) using a standard curve for each oligosaccharide study is in agreement with others showing the synergistic effect of Abf and cellulose/hemicellulose. The Novozymes ULTRAFLO XL has not only Abf, but also xylanase, pentosanase, cellulase, and α-amylase activity. Probably this explains the greater efficiency of the Novozymes ULTRA-FLO XL observed in our study.

Discussion
In the past decade, improvements in the utilization of renewable energy resources have attracted great interest worldwide [21]. The use of waste by-products of agricultural cereals as important sources of biomass helps avoid competition between energy and food crops [1]. For example, the by-product stream from wheat contains ~ 66% arabinoxylan by weight, of which ~ 74-91% by weight is water-soluble [22]. To increase the utilization of arabinoxylan in the waste by-products of agricultural cereals, it is important to efficiently degrade the abundant arabinoxylan into its monosaccharides, arabinose and xylose. In this study, a GH51 Abf TlAbf51 was identified from T. leycettanus JCM12802, and its high expression was achieved in P. pastoris GS115. As shown in Table 1, the optimal pH (3.5-4.0) for this enzyme was similar to that for Abf from A. kawachii [10], but lower than those for Abfs from C. lucknowense C1 (5.0; [13]), P. chrysogenum 31B (5.0; [14]) and Penicillium purpurogenum (5.0; [23]). TlAbf51 exhibited good stability over a wider pH range (3.0-9.0) than the other fungal GH51 Abfs. Examination of the optimal temperature and thermostability showed that TlAbf51 exceeded the general properties of formerly reported counterparts that were more active at 40-60 °C and stable at 50 °C. However, compared to the other characterized fungal Abfs, TlAbf51 exhibited Fig. 3 Combined activities of TlAbf51 with the bifunctional xylanase/cellulase TcXyn10A for water-soluble wheat arabinoxylan degradation. We hydrolyzed 0.5% wheat arabinoxylan using different enzyme combinations, including a reaction with a TlAbf51 alone, b TcXyn10A alone, c sequential reactions of TlAbf51 and TcXyn10A for 12 h, respectively, d sequential reactions of TcXyn10A and TlAbf51 for 12 h, respectively, and e simultaneous incubation with TlAbf51 and TcXyn10A for 12 h. The hydrolysates were analyzed by HPAEC-PAD significantly greater catalytic efficiency (k cat /K m value; approximately 125-fold higher) towards 4-nitrophenylα-l-arabinofuranoside. These characteristics make TlAbf51 one of the most feasible candidates for industrial applications.
In terms of its substrate specificity, TlAbf51 was highly specific for α-l-arabinofuranosyl linkages, as observed for homologous enzymes. TlAbf51 produced arabinose as the sole hydrolysis product towards sugar beet arabinan and debranched sugar beet arabinan, but the amount Fig. 4 Synergistic effects of TlAbf51 with the bifunctional xylanase/cellulase TcXyn10A. a Reducing sugars obtained by a reaction with TlAbf51 or TcXyn10A, singly and in combination with different enzyme-activity ratios. b Degree of synergy observed after the combined reaction with TcXyn10A and TlAbf51. The synergic degree was defined as the amounts of saccharides released from the simultaneous activities of both enzymes to the sum of those released by both enzymes individually liberated from the former was significantly higher than from the latter (480.7 vs. 64.4 mg/L). This suggests that TlAbf51 is a type B Abf, given that type B Abfs are more active against polymeric arabinoxylan, which is singly or doubly substituted with 1,2-or 1,3-linked arabinose residues [24]. Two recent reports described bacterial GH51 Abfs isolated from Alicyclobacillus sp. A4 (AcAbf51A; [25]) and Paenibacillus sp. THS1 (THSAbf; [26]) that displayed both Abf and xylanase activities. However, 12-h digestion of water-soluble wheat arabinoxylan with TlAbf51 generated arabinose as the sole product (Fig. 3a), and no xylooligosaccharides were produced. To date, no studies have demonstrated that fungal GH51 Abfs exhibit xylanase activity. The deduced amino acid sequence of TlAbf51 shares 23.5% similarity with those of AcAbf51A and THSAbf (Additional file 7). Bouraoui et al. [25] demonstrated the functional importance of the catalytic dyad (Glu177 and Glu296) and hydrophobic residue Trp101 located on the β2α2 loop in THSAbf; the corresponding residues in TlAbf51 are Gln199, Pro354, and Gly120, respectively. These results indicate that the function of fungal Abf is distinct from that of bacterial Abf.
To improve the hydrolysis efficiency for xylan, combinations of TlAbf51 and bifunctional xylanase/cellulase TcXyn10A were examined both sequentially and simultaneously. Significant synergy occurred in all sequential reactions performed with different enzyme-activity ratios, with the highest arabinose and xylooligosaccharides production observed when TlAbf51 was added after TcXyn10A or when both enzymes were added simultaneously (Fig. 3). Similar observations have been reported for AcAbf51A [24]. These results indicate that efficient degradation of wheat arabinoxylan occurred by first interrupting the main chains with TcXyn10A, followed by branch separation with TlAbf51. The underlying mechanism may involve cleavage of the main chains by TcXyn10A which can make arabinobranched xylooligosaccharides more accessible to TlAbf51, while removal of the side chains of arabino-branched xylooligosaccharides by TlAbf51 can also improve the degradation efficiency of arabino-branched xylooligosaccharides by TcXyn10A [27]. Based on this, we tested nine enzymeactivity ratios with TcXyn10A and TlAbf51 and compared their performances in wheat arabinoxylan degradation. The combination of TcXyn10A and TlAbf51 at an enzymeactivity ratio of 1:5 was determined to be the most efficient (Fig. 4). It is well-known that it is difficult to degrade complex substrates such as wheat arabinoxylan which contains large amounts of arabinoxylan [28,29]. Arabinobranched polysaccharides and xylooligosaccharides were efficiently debranched by TlAbf51. Interestingly, similar activity ratios between core and accessory enzymes utilized for natural substrate degradation have been reported, such as those for polygalacturonase and pectin methylesterase from Penicillium oxalicum for pectin degradation [30], as well as endoglucanase and cellobiohydrolase from Irpex lacteus for cellulose degradation [31]. Therefore, the Hydrolysis of sodium hydroxide-pretreated cornstalk and corn bran with TlAbf51 and TcXyn10A. a Reducing sugars obtained by a reaction with TlAbf51 or TcXyn10A individually or in combination at an enzyme-activity ratio of 1:5 for 3 h. Oligosaccharides were obtained by degradation of hydroxide-pretreated cornstalk (b) and corn bran (c) in synergistic hydrolysis assays performed for 3, 6, 9, 12, 24 and 36 h ideal enzyme-activity ratio (between core and accessory enzymes) for maximizing hydrolysis yields and minimizing enzyme usage in biomass degradation may be approximately 1:5. Using this enzyme-activity ratio, 1.9 and 2.8 g/L of oligosaccharides were obtained after 24-h hydrolysis of cornstalk and corn bran, respectively.

Conclusions
An Abf from T. leycettanus JCM12802 was heterologously expressed and characterized. The acidity of TlAbf51 exhibited good stability over a broad pH range (3.0-9.0), and TlAbf51 exhibited significantly greater catalytic efficiency than other fungal GH51 Abfs. The enzyme preferentially removed 1,2-or 1,3-linked arabinose residues from arabinoxylan and acted synergistically with the bifunctional xylanase/cellulase TcXyn10A. Simultaneous addition of TcXyn10A and TlAbf51 resulted in the highest degradation efficiency of wheat arabinoxylan at an enzyme-activity ratio of 1:5. Additionally, this enzyme cocktail exhibited efficient degradation of sodium hydroxide-pretreated cornstalk and corn bran. This study demonstrated the efficient enzymatic saccharification of lignocellulose and suggests the high potential of using TlAbf51 in the field of biomass pretreatment.

Strains, vectors, and media
Talaromyces leycettanus JCM12802 (Japan Collection of Microorganisms RIKEN BioResource Center, Tsukuba, Japan) was cultured in medium containing lignocellulose as the sole carbon source at 45 °C for 3 days [32]. Escherichia coli strain Trans I-T1 and the pEASY-T3 vector (TransGen, Beijing, China) were employed for DNA manipulation. P. pastoris GS115 and the pPIC9 vector (Invitrogen, Carlsbad, CA, USA) were used for heterologous gene expression.

Cloning Tlabf51
The full-length Tlabf51 gene was identified in the genome of T. leycettanus strain JCM12802 (whole genome sequenced, unpublished). After growth for 3 days in induction medium, mycelia were collected to extract total RNA, which were further purified using the SV Total RNA isolation system (Promega, Madison, WI, USA). First-strand cDNA was generated with the Rever-Tra Ace-α-® Kit (TOYOBO, Osaka, Japan) using purified total RNA as a template. Subsequently, the full-length cDNA of Tlabf51 was amplified by high-fidelity PCR using specific primers (no signal peptide coding sequence based on SignalP 4.0 prediction. F: 5′-ATG AAA ACC CTC CCC GCA TTG GCC GGC GGC -3′; and R: 5′-CTA AGA CAC GGC CAG CAC CGC AAC AGC CCA -3′). Next, the specific gene fragment was cloned into the pEasy-T3 vector for sequencing.

Heterologous expression and purification
The cDNA fragment encoding Tlabf51 was amplified from the pEasy-T3-Tlabf51 plasmid using primers with flanking restriction sites (pF: 5′-TTGAA TTC ATG AAA ACC CTC CCC GCA TTGG-3′; and pR: 5′-TAGCG GCC GCC TAA GAC ACG GCC AGC ACC G-3′; EcoRI and NotI sites underlined, respectively). The PCR product was gelpurified, digested with corresponding restriction endonucleases, and then linked into the vector pPIC9. After verification by DNA sequencing, BglII was used to linearize the recombinant plasmid followed by electroporation to transform P. pastoris GS115 competent cells. Based on enzymatic activities in shake tubes, the positive transformants were screened, and the transformant with the highest Abf activity was selected for fermentation following as described by Yang et al. [27].
To remove cell debris and undissolved materials, the induced cultures were collected and centrifuged at 12,000×g for 10 min at 4 °C. The cell-free culture supernatant was concentrated with a 10-kDa molecular weight cutoff Vivaflow 200 membrane (Vivascience, Hannover, Germany), followed by desalination in 20 mM McIlvaine buffer (pH 3.0) using a 5-mL HiPrep desalting column. Next, the desalted sample was loaded onto a HiTrap SP HP 5-mL FPLC column (GE Healthcare), which had been pre-equilibrated with McIlvaine buffer. To obtain the target proteins, a linear gradient of NaCl (0-1.0 M) in the same buffer was used. Fractions showing enzyme activities were eluted and subjected to SDS-PAGE. The protein concentration was measured by the Bradford assay via determining the absorbance at 595 nm. Bovine serum albumin was used as the standard.

Enzyme assay
Abf activity was determined according to the method of Yang et al. [27], with some modifications. Briefly, standard reactions contained 250 µL of 1 mM 4-nitrophenyl-αl-arabinofuranoside and 250 μL properly diluted enzyme solution in 0.1 M McIlvaine buffer (pH 3.5). After incubation at 50 °C for 10 min, 1.5 mL 1 M Na 2 CO 3 was added to terminate the reaction. The absorption at 405 nm was determined to detect the amount of p-nitrophenol released. All reactions were performed in triplicate. One unit of Abf activity was defined as the amount of enzyme that released 1 μmol of 4-nitrophenyl/min under standard conditions. Xylanase activity was measured using the DNS method [33] with d-xylose as the standard. The reaction system containing 900 μL 1% (w/v) water-soluble wheat arabinoxylan (Megazyme) in 0.1 M McIlvaine buffer (pH 3.5) and 100 μL of an appropriately diluted enzyme solution was incubated at 55 °C for 10 min followed by the addition of 1.5 mL DNS reagent, and then the concentration of reducing sugars was determined by measuring the absorption at 540 nm. Each reaction was performed in triplicate. One unit of xylanase activity was defined as the amount of enzyme that released reducing sugars equivalent to 1 μmol of d-xylose/min under the assay conditions.

Biochemical characterization
The pH optima in terms of the activity of purified recombinant TlAbf51 was measured in 10-min reactions performed at 55 °C in 0.1 M McIlvaine buffer over a pH range of 2.5-7.0. To estimate enzyme stability at different pH levels (0.1 M McIlvaine buffer, pH 2.5-7.0; 0.1 M Tris-HCl, pH 8.0-9.0), residual activities were measured under standard conditions after the enzyme was preincubated in buffer without substrate at 37 °C for 1 h. To determine the optimum reaction temperature, 10-min reactions were performed at different temperatures ranging from 30 to 70 °C at pH 3.5. The thermal stability of TlAbf51 was investigated by measuring residual activities under standard conditions after pre-incubation of the enzyme for 30 min at the same temperatures (as described above) in the absence of substrate.
Enzyme-kinetics assays were determined at 55 °C for 5 min in 0.1 M McIlvaine buffer (pH 3.5) with 0.1-5 mM 4-nitrophenyl-α-l-arabinofuranoside as substrate. The constants (K m and V max values) of TlAbf51 were plotted by fitting the data to a Michaelis-Menten plot using GraphPad Prism software (GraphPad, Inc., La Jolla, CA, USA).

Synergistic hydrolysis of wheat arabinoxylan with TlAbf51 and TcXyn10A
The bifunctional xylanase/cellulase TcXyn10A from T. crustaceus JCM12803 [20] is an excellent, economically viable candidate for the enzymatic degradation of plant cell wall polysaccharides for biofuels and bio-based chemicals. Thus, its synergistic activity with TlAbf51 in the hydrolysis of wheat arabinoxylan was investigated. To study the hydrolytic activities of TlAbf51 and TcXyn10A on water-soluble wheat arabinoxylan, the hydrolysis products were analyzed by HPAEC-PAD as described above. All reaction systems containing 900 μL of 0.5% (w/v) substrate and 100 μL of enzyme(s) (0.5 U each of TlAbf51 and/or TcXyn10A) were performed at 37 °C in 0.1 M McIlvaine buffer (pH 4.0). After 12-h incubation, the reactions were terminated by heat denaturation by boiling for 10 min. The second enzyme solution was then added for the sequential reactions. The reaction system with substrate but without any enzyme was defined as the blank control. The resulting hydrolysis products were analyzed by the HPAEC-PAD method.
To determine the extent of synergy, different enzymeactivity ratios were used, and the production of reducing ends from water-soluble wheat arabinoxylan was measured. Experimentally, 0.5 U of TcXyn10A was combined with TlAbf51 at enzyme-activity ratios ranging from 1:1 to 1:10 and incubated with 0.5% wheat arabinoxylan. The hydrolysis reactions were carried out in McIlvaine buffer at pH 4.0 and 37 °C for 12 h, and then the reactions were terminated by heat denaturation by boiling for 10 min. The amount of reducing sugars released was determined using the DNS method.

Synergistic hydrolysis of sodium hydroxide-pretreated cornstalk and corn bran
Cornstalk and corn bran pretreatments were performed according to Zhuo et al. [5]. The milled cornstalk and corn bran samples were autoclaved at 120 °C for 1 h with 1% (w/v) sodium hydroxide at a 10% ratio (w/v). Next, pretreated samples were filtered through eight layers of gauze, and then washed multiple times with distilled water, followed by drying in a thermotank at 40 °C to achieve a constant weight for subsequent saccharification experiments. Synergistic hydrolysis of sodium hydroxide-pretreated cornstalk and corn bran was studied in 0.1 M McIlvaine buffer (pH 4.0) containing pretreated samples (0.5%, w/v), 0.5 U of TcX-yn10A, and 2.5 U of TlAbf51. The reaction system with substrate but without any enzyme was defined as the blank control. Hydrolysis proceeded for various durations, and the samples were collected and analyzed by HPAEC-PAD.