Fermentative production and direct extraction of (−)-α-bisabolol in metabolically engineered Escherichia coli

Background (−)-α-Bisabolol, also known as levomenol, is an unsaturated sesquiterpene alcohol that has mainly been used in pharmaceutical and cosmetic products due to its anti-inflammatory and skin-soothing properties. (−)-α-Bisabolol is currently manufactured mainly by steam-distillation of the essential oils extracted from the Brazilian candeia tree that is under threat because its natural habitat is constantly shrinking. Therefore, microbial production of (−)-α-bisabolol plays a key role in the development of its sustainable production from renewable feedstock. Results Here, we created an Escherichia coli strain producing (−)-α-bisabolol at high titer and developed an in situ extraction method of (−)-α-bisabolol, using natural vegetable oils. We expressed a recently identified (−)-α-bisabolol synthase isolated from German chamomile (Matricaria recutita) (titer: 3 mg/L), converted the acetyl-CoA to mevalonate, using the biosynthetic mevalonate pathway (12.8 mg/L), and overexpressed farnesyl diphosphate synthase to efficiently supply the (−)-α-bisabolol precursor farnesyl diphosphate. Combinatorial expression of the exogenous mevalonate pathway and farnesyl diphosphate synthase enabled a dramatic increase in (−)-α-bisabolol production in the shake flask culture (80 mg/L) and 5 L bioreactor culture (342 mg/L) of engineered E. coli harboring (−)-α-bisabolol synthase. Fed-batch fermentation using a 50 L fermenter was conducted after optimizing culture conditions, resulting in efficient (−)-α-bisabolol production with a titer of 9.1 g/L. Moreover, a green, downstream extraction process using vegetable oils was developed for in situ extraction of (−)-α-bisabolol during fermentation and showed high yield recovery (>98%). Conclusions The engineered E. coli strains and economically viable extraction process developed in this study will serve as promising platforms for further development of microbial production of (−)-α-bisabolol at large scale. Electronic supplementary material The online version of this article (doi:10.1186/s12934-016-0588-2) contains supplementary material, which is available to authorized users.

(−)-α-Bisabolol is an unsaturated sesquiterpene alcohol that occurs naturally in the Brazilian candeia tree (Eremanthus erythropappus) and in medicinal herbs such as German chamomile (Matricaria recutita) [9,10]. It has been shown to have pharmaceutical functions (e.g., antibacterial, antiseptic, and anti-inflammatory activities), and skin-soothing and -moisturizing properties [11][12][13]. Owing to the low toxicity associated with (−)-α-bisabolol, the Food and Drug Administration (FDA) has granted it the Generally Regarded as Safe (GRAS) status that has promoted its use as an active ingredient in several commercial products [14]. At present, natural (−)-α-bisabolol is primarily manufactured through the steam-distillation of candeia essential oils extracted from the Brazilian candeia tree, which has raised environmental and bioconservation issues in recent years [10]. (−)-α-Bisabolol can also be chemically synthesized; however, chemical synthesis requires an additional economically unviable purification step because of the formation of other diastereomers ((+)-α-bisabolol and (±)-epi-α-bisabolol) and undesirable byproducts [15]. Therefore, a sustainable supply of nature-identical (−)-α-bisabolol is essential to specialty chemical industries. Recently, a (−)-α-bisabolol synthase capable of synthesizing enantio-selective (−)-α-bisabolol as a single terpenoid product has been identified in German chamomile. (−)-α-bisabolol synthase was expressed in Saccharomyces cerevisiae for the production of (−)-α-bisabolol and had a yield of 8 mg/L after 4 days of cultivation [15]. This previous study has paved the way for the biological production of enantioselective (−)-α-bisabolol in engineered microorganisms, but the productivity is very low for industrial production of (−)-α-bisabolol.
n-Dodecane was used as an overlay to prevent the loss of volatiles. Furthermore, n-dodecane was used to solubilize and extract (−)-α-bisabolol, which is toxic to E. coli cell growth in high concentrations, from the culture media during cultivation. MVA was prepared from mevalonolactone (Sigma Aldrich) as previously described [16]. Cell growth was monitored by measuring the optical density at a wavelength of 600 nm (OD 600 ) with a spectrophotometer (Ultrospec 8000, GE Healthcare, Uppsala, Sweden). The inhibitory effect of (−)-α-bisabolol on the growth of E. coli strains was investigated in LB liquid medium supplemented with various concentrations of (−)-α-bisabolol. Diluted samples from cultures during the stationary phase were plated on LB solid medium and the colony-forming units (CFUs) were determined after overnight incubation at 30 °C.

Plasmid construction
Plasmids and polymerase chain reaction (PCR) primers used in this study are listed in Table 1. Common procedures, including genomic DNA preparation, restriction digestions, transformations, and other standard molecular biological techniques were performed as previously described [17]. All restriction enzymes and T4 DNA ligase were purchased from New England Biolabs (Ipswich, USA). PCR was performed following the manufacturer's instructions, using a high fidelity KOD-Plus-Neo polymerase (Toyobo, Osaka, Japan). Plasmid preparation and gel extraction kits were obtained from Promega (Madison, USA) and oligonucleotides were synthesized by Bioneer (Daejeon, Korea). The MrBBS gene (GenBank accession number: KJ020282) was optimized to E. coli codons and synthesized by Bioneer (Additional file 1: Figure S1). We deposited the nucleotide sequence data of E. coli codon-optimized MrBBS in the GenBank database (accession number: KU680479). The synthesized MrBBS was amplified by PCR with Bis-IF and Bis-IR primers (Table 1) and the plasmid backbone was amplified with the Bis-VF and Bis-VR primers from pTrc99A (GE Healthcare). The two PCR-amplicons were assembled via the Gibson Assembly Method [18] using Gibson Assembly Master Mix (New England Biolabs), to produce the pTSN-MrBBS plasmid. The entire polycistronic MVA pathway genes were inserted into pTSN-MrBBS through XbaI restriction site digestion of both the pTSN-MrBBS and the pSNA plasmids containing all the mevalonate pathway genes [19], followed by ligation with T4 DNA ligase. The resultant plasmid was designated pSNA-MrBBS. To overexpress FPP synthase, the ispA gene (GenBank accession number: AAC73524) was PCR-amplified from E. coli MG1655 genomic DNA, using IspAI-F and IspAI-R primers. To avoid proofreading errors introduced during PCR, the amplified ispA gene was first inserted into the XbaI restriction site of pTSN-MrBBS (instead of pSNA-MrBBS). The pTSN-MrBBS-IspA and pSNA plasmids were digested with XbaI followed by ligation with T4 DNA ligase to create pSNA-MrBBS-IspA. The direction of the MVA pathway genes was verified by Sanger sequencing.

Batch and fed-batch fermentation
To prepare the pre-culture, the engineered E. coli was cultured in 100 mL of TB medium at 30 °C and 180 rpm. The batch fermentation was performed by inoculating 1% (v/v) of pre-culture into 400 mL TB medium supplemented with 10 g/L glycerol and overlaid with 80 mL n-dodecane in a 1 L fermenter. The cultures were incubated at 30 °C and air-aerated with a flow rate of 1 volume of air per volume of medium per min (vvm), the agitation speed remained fixed at 200 rpm in all experiments. Glycerol was used as a carbon source at a concentration of 10 g/L. The pH was balanced to pH 7.0 by the addition of 1 M HCl and 25% NH 4 OH solutions. n-Dodecane was overlaid up to 20% (v/v) of culture volume to continuously extract (−)-α-bisabolol from the culture broth during fermentation. The fed-batch fermentation began as a batch operation and was switched to the fed-batch mode at 24 h, when OD 600 reached 3.0. At the beginning of fedbatch fermentation, TB medium was supplemented with 10 g/L glycerol, 0.1% (v/v) trace element solution (27 g/L FeCl 3 ·6H 2 O, 2 g/L ZnCl 2 ·4H 2 O, 2 g/L CoCl 2 ·6H 2 O, 2 g/L Na 2 MoO 4 ·2H 2 O, 1 g/L CaCl 2 ·2H 2 O, 1.3 g/L CuCl 2 ·6H 2 O, and 0.5 g/L H 3 BO 3 ) and 1% (v/v) vitamin solution (Sigma Aldrich, Cat. No. M6895). Glycerol was then fed at a rate

Table 1 List of strains, plasmids, and primers used in this study
Overlap region for Gibson Assembly is underlined a Primer sequences are indicated in the 5′-3′ direction

(−)-α-Bisabolol extraction with n-dodecane and vegetable oils
Extraction of (−)-α-bisabolol was performed using four common vegetable oils: canola, olive, corn, and soybean oil. A total of 0.1 g of (−)-α-bisabolol was added to 10 mL of sterile TB medium in a glass vial and 1 mL of (Agilent, Santa Clara, USA). The quantification of (−)-α-bisabolol was performed using GC equipped with a flame ionization detector (FID) with a HP-5 column (30 m × 0.320 mm × 0.25 µm) at a flow rate of 1 mL/min. The starting temperature of the oven was 60 °C for 2 min, increased at 5 °C/min to 200 °C, held at 200 °C for 2 min, increased by 50 °C/min to 300 °C, and held at 300 °C for 5 min. Helium was used as the carrier gas with an inlet pressure of 5.58 psi. The (−)-α-bisabolol concentration produced by engineered E. coli was determined as follows: individual vegetable oil was overlaid to the solution. After gentle stirring of the mixture, it was incubated at 25 °C for 1 h to separate the water-based TB medium and oil phase. The aqueous layer was removed and the organic layer was transferred to a new vial for gas chromatography (GC) analysis of (−)-α-bisabolol. The recovery yield of each vegetable oil was compared with that of n-dodecane. To determine the recovery efficiency of n-dodecane from cell pellets and TB medium, E. coli DH5α cells transformed with the pSNA-MrBBS-IspA plasmid were cultivated in TB medium in the presence of 20% (v/v) n-dodecane at 30 °C at 180 rpm for 48 h. After centrifugation of the culture at 14,000 rpm for 10 min, the pellet, supernatant, and organic layers of n-dodecane were fractionated. To determine the recovery yield of n-dodecane, the organic layer was directly subjected to GC analysis of (−)-α-bisabolol. The isolated supernatant was mixed with 20% (v/v) n-dodecane, incubated for 30 min, and centrifuged at 14,000 rpm for 10 min. The organic layer was isolated and used for GC analysis of (−)-α-bisabolol. The cell pellet was first disrupted by sonication and the lysate was used for the extraction and quantification of (−)-α-bisabolol through the process described above.

Analysis of glycerol consumption and byproduct formation
The culture broth was centrifuged and the supernatant was used to quantify glycerol consumption and byproduct formation. Glycerol, acetate, and mevalonate concentrations were determined by high performance liquid chromatography (HPLC, Shimadzu, Kyoto, Japan) with a refractive index detector at 454 nm using Aminex HPX-87H column (1300 mm × 7.8 mm, Bio-Rad, Hercules, USA). Sulfuric acid (0.4 mM) was used as the mobile phase at a flow rate of 0.3 mL/min at 50 °C.

(−)-α-Bisabolol production in E. coli DH5α expressing the MrBBS gene
To implement the de novo production of (−)-α-bisabolol in E. coli DH5α, the gene of MrBBS, encoding the (−)-α-bisabolol synthase, was codon-optimized for E. coli and introduced via the pTSN-MrBBS plasmid (Fig. 1b). The resulting transformant cells were cultured in LB medium overlaid with 20% (v/v) n-dodecane for 48 h. Two-phase cultures have been successfully used to extract toxic, water-immiscible or volatile products [24][25][26] and n-dodecane was successfully used for extraction of (−)-α-bisabolol from the culture broth of S. cerevisiae [15]. As expected, in the present study, (−)-α-bisabolol was extracted into the n-dodecane phase in the two-phase E. coli culture. As the (−)-α-bisabolol was recovered in n-dodecane without significant residual amounts left in the cells (Additional file 1: Figure S2), (−)-α-bisabolol purification in a later step will be facilitated. The n-dodecane phase was used for GC analysis to measure (−)-α-bisabolol concentrations. There was a peak at 25.6 min in the n-dodecane phase sample of E. coli DH5α with pTSN-MrBBS, corresponding to the standard (−)-α-bisabolol compound dissolved in n-dodecane (Fig. 3a). Mass spectrometry confirmed that the peak at 25.6 min was (−)-α-bisabolol (Fig. 3b, c) and a maximum concentration of 3 mg/L (−)-α-bisabolol was produced by the endogenous MEP pathway and exogenous MrBBS enzyme in E. coli DH5α. The peak was not observed in two-phase cultures of DH5α cells containing an empty plasmid as a control experiment (Fig. 3a). The (−)-α-bisabolol synthase gene, MrBBS, used in this study was recently isolated from German chamomile and, unlike other TSPs, exclusively synthesizes (−)-α-bisabolol as a single terpene product [15]. Several TPSs producing α-bisabolol as a single major product have been previously cloned and biochemically characterized. However, these enzymes usually produce undesirable isomers with different structures or α-bisabolol of unknown stereochemistry [27][28][29]. The MrBBS enzyme has shown unique catalytic features including the formation of a single enantiopure (−)-α-bisabolol indicating the possibility of a biotechnological application to natural (−)-α-bisabolol production in S. cerevisiae [15]. Here, we have expressed the codon-optimized synthetic MrBBS in E. coli and successfully produced (−)-α-bisabolol (titer: 3 mg/L). A similar result has been observed when attempting to express other plant terpene synthases in E. coli [30,31].

(−)-α-Bisabolol production in E. coli DH5α expressing MrBBS and MVA pathway genes
Terpenoid production in microbes is mainly limited by the flux from intermediates of central metabolism (acetyl-CoA for MVA pathway or pyruvate and glyceraldehyde-3-phosphate for MEP pathway) to the substrates of TPSs (GPP, FPP, or GGPP) [32]. The introduction and engineering of the biosynthetic MVA pathway in E. coli have improved the production of terpenoids such as amorphadiene [32], carotenoids [19,21], coenzyme Q10 [33], and farnesol [30] by efficiently supplying IPP and DMAPP. Based on these studies, we exploited the biosynthetic MVA pathway for the biosynthesis of (−)-α-bisabolol in E. coli. To increase carbon flux towards TPS substrates, a plasmid (pSSN12Didi) containing the lower MVA pathway   (Fig. 1b), leading from MVA to IPP and DMAPP [30], was introduced into E. coli DH5α expressing the MrBBS. MVA is an exogenous substrate for E. coli, so supplemented MVA can only be converted to IPP and DMAPP by the engineered MVA lower pathway in E. coli. Different concentrations of MVA, ranging from 0 to 10 mM, were examined to determine whether MVA availability limits (−)-α-bisabolol production in LB medium. (−)-α-bisabolol production increased as the concentration of the supplied MVA increased (Fig. 4a) and cell growth was not significantly inhibited (Fig. 4b).
With the addition of 10 mM MVA, (−)-α-bisabolol production was 10.5 mg/L, approximately 3.5-fold higher than that obtained without the addition of MVA (3 mg/L, Fig. 4a). Expression of the lower MVA pathway genes in E. coli improved (−)-α-bisabolol production with the addition of MVA. However, MVA is not an economically viable substrate. To achieve (−)-α-bisabolol production from cost-effective renewable resources such as glycerol, a major byproduct of the biodiesel industry, we constructed a plasmid pSNA-MrBBS (Fig. 1b). pSNA-MrBBS harbors the complete biosynthetic MVA pathway genes, including the MrBBS gene, and enables production of IPP and DMAPP in the absence of MVA. A total of 12.8 mg/L of (−)-α-bisabolol was produced from glycerol in E. coli DH5α with pSNA-MrBBS grown in LB (Fig. 5a). This is a slightly higher concentration than that produced in DH5α containing only MrBBS and lower MVA pathway genes with the addition of 10 mM MVA (10.5 mg/L). Overall, the expression of biosynthetic MVA pathway genes significantly improved (−)-α-bisabolol production in E. coli.

Optimization of (−)-α-bisabolol production in E. coli DH5α expressing MrBBS and entire MVA pathway genes
IPP and DMAPP, precursors of FPP, are essential metabolites in E. coli that are used for tRNA prenylation, synthesis of quinone and dolichol for respiration, and cell wall biosynthesis [31]. Thus, the metabolic balance of IPP and DMAPP between endogenous essential metabolism and exogenous (−)-α-bisabolol production plays a crucial role in improving production of (−)-α-bisabolol and alleviation of growth inhibition. To determine the optimal IPP and DMAPP pool balancing conditions in the engineered E. coli harboring the entire MVA pathway and MrBBS genes (pSNA-MrBBS), we examined if cultivation media and glycerol availability limit (−)-α-bisabolol production. To do this we tested different media and concentrations of glycerol ranging from 0 to 3.5% (w/v) (Fig. 5a). E. coli DH5α containing the pSNA-MrBBS produced the highest concentration of (−)-α-bisabolol (24.2 mg/L) in TB medium without the addition of an additional carbon source (Fig. 5a). (−)-α-Bisabolol production was dependent on the amount of provided glycerol in the TB medium. With 1% (w/v) glycerol, (−)-α-bisabolol concentration was 38.3 mg/L, approximately 1.4-fold higher than the concentration of (−)-α-bisabolol obtained without the inclusion of glycerol. However, 3.5% (w/v) of glycerol concentration did not show further improvement of (−)-α-bisabolol production (Fig. 5a). Because cell growth of E. coli DH5α varied in different culture media, the differences in (−)-α-bisabolol production are partly due to differences in growth ( Figure S3A). Plasmid pSNA-MrBBS contained IPTG-inducible lac and trc promoters for expression of the complete MVA pathway genes and MrBBS gene, respectively (Fig. 1b). To ascertain the optimal inducer concentration for (−)-α-bisabolol production in E. coli DH5α with pSNA-MrBBS, induction was conducted with varying concentrations of IPTG ranging from 0 to 0.1 mM. Interestingly, leaky expression of the MrBBS and MVA pathway genes in the absence of IPTG showed the highest production of (−)-α-bisabolol (38.9 mg/L). At all other IPTG concentrations (−)-α-bisabolol production decreased as the IPTG concentration increased (Fig. 5b) whereas the growth of E. coli DH5α cells is similar regardless of IPTG concentration (Additional file 1: Figure S3B). This result is compatible with a previous report [31] that showed lycopene production in E. coli expressing lower MVA pathway genes regulated by the trc promoter decreased under all IPTG-induced conditions. In addition, constitutive promoters controlling the expression of MVA pathway genes showed much lower production of amorphadiene than that from IPTG-inducible lacUV5 and trc promoters [34]. High expression of the coli cells grown on TB medium supplemented with 10 g/L glycerol; c pH. Different initial pHs were tested to find an optimal pH for (−)-α-bisabolol production. E. coli cells were cultivated in TB medium containing 10 g/L glycerol in the absence of IPTG; d E. coli strains. E. coli cells were grown on TB medium, pH 7.0 with 10 g/L glycerol and 20% (v/v) n-dodecane in the absence of IPTG. Data represent averages from three replicate cultures and error bars show SD MVA pathway and MrBBS genes under IPTG induced conditions could cause a deficiency of FPP for essential cellular metabolism. Furthermore, the accumulation of toxic intermediates of the MVA pathway, including IPP and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) [32,35], could cause growth inhibition along with a decrease of (−)-α-bisabolol production. In addition, we investigated the effects of initial pH of the cultivation of E. coli DH5α with pSNA-MrBBS on the production of (−)-α-bisabolol. The optimal pH for (−)-α-bisabolol production was pH 7 (38.2 mg/L, Fig. 5c). To determine the ideal E. coli strains for (−)-α-bisabolol production under the optimized culture conditions, we individually introduced the plasmid pSNA-MrBBS into three different E. coli strains (DH5α, MG1655, or BL21(DE3)) and measured the (−)-α-bisabolol concentration produced from each. E. coli DH5α cells produced the highest amount of (−)-α-bisabolol, and BL21(DE3) showed an approximate 13-fold decrease in (−)-α-bisabolol production compared to DH5α (38.4 mg/L, Fig. 5d). We also measured cell growth during examination of pH effects on (−)-α-bisabolol production in E. coli DH5α expressing MrBBS and entire MVA pathway genes. Although cell growth slightly decreased at a pH 5 or 6, it was similar at all other pHs (Additional file 1: Figure S3C).

Effect of the overexpression of FPP synthase on (−)-α-bisabolol production
(−)-α-Bisabolol is produced from depyrophosphorylation of the prenyl diphosphate precursor FPP. Therefore, synthesis of FPP from IPP and DMAPP by FPP synthase, encoded by the ispA gene, plays a key role in the improved production of (−)-α-bisabolol. Furthermore, high concentrations of prenyl diphosphates, such as FPP, are toxic to E. coli cell growth [36]. FPPs needs to be efficiently converted into downstream products by TPSs to reduce its toxicity. To increase the synthesis of the cellular terpenoid biosynthesis intermediate FPP, and (−)-α-bisabolol production, overexpression of an FPP synthase, encoded by the ispA gene, was performed. The ispA gene of E. coli was inserted downstream of the MrBBS gene in the plasmid pSNA-MrBBS, resulting in the pSNA-MrBBS-IspA plasmid (Fig. 1b). This plasmid was transformed into E. coli DH5α and cultivated in TB medium containing 1% (w/v) glycerol at 30 °C for 48 h using a two-phase culture without the addition of IPTG. Overexpression of the ispA gene improved (−)-α-bisabolol production compared with pSNA-MrBBS alone. After 48 h, a concentration of 79.7 mg/L of (−)-α-bisabolol was obtained in a culture of DH5α with pSNA-MrBBS-IspA. This was twofold higher than the 39.7 mg/L of (−)-α-bisabolol produced by DH5α with pSNA-MrBBS alone (Fig. 6a), without significant difference in cell growth (Additional file 1: Figure S3D). This suggested that sufficient supply of FPP is important to improve (−)-α-bisabolol production in E. coli. While overexpressing the ispA gene, 0.05 mM IPTG at OD 600 ~0.5 was added to induce expression of the MVA pathway and MrBBS genes. Similar to the results without ispA overexpression, (−)-α-bisabolol production decreased from 87.8 mg/L (no IPTG) to 40 mg/L (−)-α-bisabolol (0.05 mM IPTG, Fig. 6b) with no significant difference in cell growth (Additional file 1: Figure S3D). To monitor the time-course of (−)-α-bisabolol production, batch fermentation of E. coli DH5α harboring the pSNA-MrBBS-IspA plasmid, using a 1 L fermenter, was conducted at 30 °C in the absence of IPTG for 93 h (Fig. 6c). (−)-α-bisabolol production of 214 mg/L was achieved and cell growth reached an OD 600 value of 11.5 after 93 h. MVA and acetate were the main byproducts, although the acetate was consumed after glycerol depletion at 48 h. The solubility of heterologous enzymes increases at lower growth temperatures because the transcription rate is slower, leading to better protein folding in E. coli [37,38]. In addition, improved solubility of metabolic pathway enzymes at low temperature enhances the production yield [39]. Therefore, we performed the batch fermentation at 25 °C under the same conditions. Indeed, (−)-α-bisabolol production was 342 mg/L after fermentation for 93 h, approximately 1.6-fold higher than that obtained at 30 °C, and cell growth decreased by 25% (OD 600 8.7, Fig. 6d).

Extraction and fed-batch fermentation of (−)-α-bisabolol
n-Dodecane has been mostly used for the extraction of terpenoids produced in microorganisms due to its relatively low volatility, enabling continuous extraction over multiple days [40]. The (−)-α-bisabolol produced in flask culture and batch fermentation could be efficiently extracted from the broth to the overlaid n-dodecane phase. The in situ recovery of (−)-α-bisabolol from the n-dodecane phase during culture can reduce production costs by simplifying both the harvest and purification processes. However, n-dodecane is not an economically viable extracting solvent. We assessed various natural and inexpensive vegetable oils for selective (−)-α-bisabolol extraction from fermentation broth. As shown in Table 2, all tested vegetable oils showed extraction yields ranging from 96.6 to 98.8%, which are comparable to that of n-dodecane (99.5%). Among them, canola oil (98.8%) was the best extracting oil and was used for the extraction of (−)-α-bisabolol during fed-batch fermentation. A greener extraction of (−)-α-bisabolol plays a key role in the application of (−)-α-bisabolol produced from fermentation broth to pharmaceutical and cosmetic products. We successfully used vegetable oils as natural, cost-effective, and biodegradable extractors for in situ extraction of (−)-α-bisabolol during fermentation. Moreover, due to the difference in polarity between the fermentation broth (water based) and vegetable oils, there is a phase separation, resulting in isolation of oil from the broth containing cell debris. This in situ (−)-α-bisabolol separation from the fermentation broth can also alleviate production inhibition and improve (−)-α-bisabolol production, which will be of interest in industrial processes. To examine the production performance of (−)-α-bisabolol under conditions more relevant to industrial processes, we conducted (−)-α-bisabolol fermentation in a 50 L fermenter with a fed-batch mode. E. coli DH5α, containing pSNA-MrBBS-IspA, was grown at 25 °C with 10% (v/v) canola oil as an extracting solution. The final (−)-α-bisabolol titer reached 3.3 g/L after 90 h of fermentation, which was almost 8.4-fold higher than that obtained from the batch fermentation at 25 °C (Fig. 7). At the beginning of fermentation, MVA was accumulated until depletion of the initial 10 g/L glycerol. After complete depletion of initial glycerol, MVA was consumed and (−)-α-bisabolol accumulated continuously up to 9.1 g/L at the end of fermentation (Fig. 7). The specific (−)-α-bisabolol production IspA overexpression and batch fermentation of engineered E. coli. a Improvement of (−)-α-bisabolol production in E. coli DH5α transformed with pSNA-MrBBS-IspA compared with DH5α harboring the pSNA-MrBBS plasmid. Cells were cultivated in TB medium containing 10 g/L glycerol at 30 °C for 48 h using two-phase culture without the addition of IPTG. Data represent averages from three replicate cultures; error bars show SD, b effects of IPTG addition on (−)-α-bisabolol production in E. coli DH5α harboring pSNA-MrBBS-IspA. Culture conditions were the same as in Fig. 6a, except for the addition of IPTG. IPTG was added to each culture to a final concentration of 0.05 mM when the OD 600 reached 0.5. Data represent averages from three replicate cultures; error bars show SD, c time profile of batch fermentation of E. coli DH5α with pSNA-MrBBS-IspA in TB medium containing 10 g/L of glycerol as carbon source at 30 °C, n-dodecane was overlaid up to 20% (v/v) of culture volume to continuously extract (−)-α-bisabolol from the culture broth during fermentation. d Time profile of batch fermentation of E. coli DH5α with pSNA-MrBBS-IspA in TB medium containing 10 g/L of glycerol as carbon source at 25 °C. n-Dodecane was overlaid up to 20% (v/v) of culture volume to continuously extract (−)-α-bisabolol from the culture broth during fermentation n-Dodecane 99.5

Fig. 7
Fed-batch fermentation of E. coli DH5α in TB medium supplemented with 10 g/L of glycerol as initial carbon source. After complete depletion of glycerol, glycerol was fed at 3 g/L/h. Concentrations of acetate, mevalonate, and glycerol were determined by HPLC and (−)-α-bisabolol content was measured using GC. Canola oil 10% (v/v) instead of n-dodecane 20% (v/v) was used to overlay 30 L TB medium in a 50 L fermenter